Western Blot of Rabbit anti-PARP1-AD with protein

Datasheet

PARP1 (internal) Control Protein

Name: PARP1 (internal) Control Protein
Brand: Rockland Immunochemicals
Availability: OutOfStock

Item No. 009-001-X51

Size: 25 µg

Applications: WB, SDS-PAGE

Origin: Human

Expressed in: E. coli

This product is discontinued

Background

PARP1 is the primary member of the poly(ADP-ribose) polymerase family, whose function is to signal DNA damage (and to recruit repair proteins) by PARylation. PARP1 is also involved in multiple cell death pathways, including apoptosis, necroptosis, autophagy, and a relatively new pathway termed parthanatos. PARP1 can also promote tissue survival by shifting the balance of cell death programs between autophagy and necrosis. Clinical studies have shown vulnerability to PARP inhibitors in DNA repair defective cancers. PARP1-AD is useful for researchers interested in cellular processes including DNA damage, transcriptional control, and stem cell identity research.

Expand Background

Specifications for PARP1 (internal) Control Protein

Product Details

Description: PARP1 (internal) Control Protein - 009-001-X51

Synonyms: Poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, PPOL

Species of Origin: Human

Expressed in: E. coli

Type: Recombinant Protein

Target Details

Gene Name: PARP1 - View All PARP1 Products

Purity/Specificity: PARP1 (internal) is a N-terminus His-Tag recombinant protein expressed in E.coli that includes the human PARP1 automodification domain and the Breast Cancer Suppressor Protein (BRCA1), carboxy-terminal domain . Analysis by SDS-PAGE and Coomassie staining resulted in ~19 kDa MW band and estimated purity ≥90%.

Database Links:

UniProtKB - P09874

Application Details

Tested Applications: SDS-PAGE, WB

Application Note: PARP1 (internal) has been tested by SDS-PAGE and western blot and is suitable as a control for immunological assays that use Anti-PARP1 (internal) (RABBIT) Antibody - 200-401-X51. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 20 kDa in size corresponding to PARP-1 by western blotting in the appropriate cell lysate or extract.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 1.0 mg/ml by modified Lowry assay

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10% (v/v) Glycerol

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable 2 - 3 weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.