Datasheet

PAD3 protein-GST fusion

Name: PAD3 protein-GST fusion
Brand: Rockland Immunochemicals
Price: 325.0 USD
Availability: OutOfStock

Item No. 009-001-S75

Size: 50 µg

Origin: Human

Expressed in: Sf9 cells

$665.00 /Per Item

20 µg $325.00

50 µg $665.00

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Background

PAD3 is a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. PAD3 modulates hair structural proteins, such as filaggrin in the hair follicle and trichohyalin in the inner root sheath, during hair follicle formation. It converts arginine to citrulline in filaggrin at a faster rate than it does in trichohyalin (1). Together with the type I enzyme (PAD1), PAD3 play a role in terminal differentiation of the epidermis. PAD3 Protein is ideal for investigators involved in Signaling Proteins, Arginine Deiminase Proteins, and Inflammation Proteins research.

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Specifications for PAD3 protein-GST fusion

Product Details

Description: PAD3 recombinant protein-GST fusion protein - 009-001-S75

Synonyms: MGC126307, MGC126308, PADI3, PDI3, Protein-arginine deiminase type-3

Species of Origin: Human

Expressed in: Sf9 cells

Type: Recombinant Protein

Target Details

Gene Name: PADI3

Purity/Specificity: Recombinant full-length human PAD3 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Database Links:

NCBI - NM_016233

Application Details

Application Note: PAD3 Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. PAD3 Protein is suitable for use in Western Blot. Expect a band approximately ~97kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 0.1 µg/µL

Buffer: See application note.

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiration: Expiration date is one (1) year from date of receipt.

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.