Mouse IgM
Mouse IgM Whole Molecule - SDS-PAGE
SDS-PAGE of Mouse IgM Whole Molecule (p/n 010-0107)
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Datasheet

Mouse IgM

010-0107
1 mg
SDS-PAGE, ELISA, IP
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Description

Background

Mouse IgM, or mouse Immunoglobulin M, purified protein is a basic antibody that is produced by B cells. Mouse IgM is by far the physically largest antibody in the human circulatory system. Mouse IgM is the first antibody to appear in response to initial exposure to antigen. IgM is predominantly produced in the spleen. Mouse IgM is formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites.

Application Note

Mouse IgM whole molecule has been tested by SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Purity/Specificity

Mouse IgM was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and anti-Mouse IgM (µ chain specific).   No reaction was observed against anti-Mouse IgG F(c).  Some light chain cross-reactivity will occur with anti-Mouse IgG.

Storage Condition

Store vial at 4° C prior to opening.   This product is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

MOUSE IgM whole molecule - 010-0107
Mouse Immunoglobulin M
SDS-PAGE
ELISA, IP
Mouse
IgM
Protein
Native Protein
Liquid (sterile filtered)
Wet Ice
Expiration date is one (1) year from date of opening.
1.0 mg/ml by UV absorbance at 280 nm
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
0.1% (w/v) Sodium Azide
None
Ardi VC et al. Macrocycles that inhibit the binding between heat shock protein 90 and TPR-containing proteins. (2011)
Applications
IP, Co-IP
Waldschmidt TJ et al. Abnormal T cell-dependent B-cell responses in SCID mice receiving allogeneic bone marrow in utero. Severe combined immune deficiency. (2002)
Applications
E, EIA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.