Mouse IgG1 isotype control Phycoerythrin
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Datasheet

Mouse IgG1 isotype control Phycoerythrin

Monoclonal MG1 IgG1

010-0840
1 mL
Dot Blot
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$261.00 /Per Item
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Description

Background

Isotype control Mouse IgG1 is important for Flow Cytometry. Mouse IgG1 control has no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.

Application Note

Mouse IgG1 isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Purity/Specificity

This product was prepared from ascitic fluid by affinity chromatography.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG1 and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. Phycoerythrin Conjugated Mouse IgG1 was proven by Dot Blot.

MOUSE IgG1 isotype control Phycoerythrin conjugated - 010-0840
Phycoerythrin conjugated Mouse IgG1 subclass isotype control, Mouse IgG1 isotype control PE conjugation
Dot Blot
R-Phycoerythrin (RPE)
Monoclonal
MG1
IgG1
Protein
Native Protein
Lyophilized
1.0 mg/mL by absorbance = 82.0 at 565 nm
0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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