Datasheet

MEK2 pS222-pS226 hu recomb protein

Name: MEK2 pS222-pS226 hu recomb protein
Brand: Rockland Immunochemicals
Availability: OutOfStock

Item No. 009-001-GQ5

Size: 10 µg

Origin: Human

Expressed in: Sf9 cells

This product is discontinued

Background

MEK2 pS222-pS226 is an activated recombinant protein was produced in Sf9 cells by co-expression of B-raf. Mitogen-activated protein kinase kinase 2, also known as MAP2K2, MEK, MEK2, MKK2 is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 2 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. This recombinant protein is the MEK2 isoform and is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Expand Background

Specifications for MEK2 pS222-pS226 hu recomb protein

Product Details

Description: MEK2 pS222-pS226 human recombinant protein - 009-001-GQ5

Synonyms: MEK2, MKK2, PRKMK2 ,CFC4, MEK-2 Antibody, MEK-2 recombinant protein

Species of Origin: Human

Expressed in: Sf9 cells

Type: Recombinant Protein

Target Details

Gene Name: MAP2K2 - View All MAP2K2 Products

PTM Specificity: Dual Modification

Purity/Specificity: MEK2 pS222-pS226 is a recombinant protein containing a polyhistidine tag expressed in Sf9. This protein is expressed in the presence of constitutively active B-raf (V600E) leading to high levels of phosphorylation of MEK2 activation residues S222 and S226. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK2 and was estimated to be greater than 90% pure.

Database Links:

UniProtKB - P36507

Application Details

Application Note: MEK2 pS222-pS226 is an activated recombinant protein suitable as a control for polyclonal or monoclonal anti-MEK2 in immunological assays. Activated MEK1 can be used in vitro to phosphorylate ERK protein. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Formulation

Physical State: Liquid (sterile filtered)

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.