MEK1 phosphoS218-pS222 hu recomb protein
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Datasheet

MEK1 phosphoS218-pS222 hu recomb protein

009-001-GP5
10 µg
Human
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Description

Background

MEK1 pS218-pS222 is an activated recombinant protein was produced in Sf9 cells by co-expression of B-raf. Mitogen-activated protein kinase kinase 1, also known as MKK or MEK, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. This recombinant protein is the MEK1 isoform and is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Application Note

MEK1 pS218-pS222 is an activated recombinant protein suitable as a control for polyclonal or monoclonal anti-MEK1 in immunological assays. Activated MEK1 can be used in vitro to phosphorylate ERK protein. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Purity/Specificity

MEK1 pS218-pS222 is a recombinant protein containing a polyhistidine tag expressed in Sf9. This protein is expressed in the presence of constitutively active B-raf (V600E) leading to high levels of phosphorylation of MEK1 activation residues at position S218 and S222. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK1 and was estimated to be greater than 90% pure.

MEK1 pS218-pS222 human recombinant protein - 009-001-GP5
MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 recombinant protein
MAP2K1
Dual Modification
Human
Sf9 cells
Recombinant Protein
Liquid (sterile filtered)
None
Dry Ice
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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