Maltose Binding Protein (MBP) Control Protein
SDS- Maltose Binding Protein (MBP) Control Protein
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Datasheet

Maltose Binding Protein (MBP) Control Protein

000-001-385-1 000-001-385-5
1 mg 5 mg
SDS-PAGE, WB
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Description

Background

Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has an approximate molecular mass of 42.5 kilodaltons. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest.

Application Note

Maltose-Binding Protein is suitable for use as a control in many assays including affinity purification, western blot and other biological assays. Optimal concentration should be determined by the researcher. Expect MW of MBP~51kDa consisting of multiple cloning sites from vector (MCS) and the MBP; molecular weight of MBP at ~40kDa. Maltose-Binding Protein has been tested in SDS-page and western blot.

Purity/Specificity

Maltose-Binding Protein Control Protein is chromatographically purified maltose binding protein.

Maltose Binding Protein (MBP) Control Protein - 000-001-385-1
control protein, MBP, Maltose Binding Protein
SDS-PAGE, WB
MBP
E. coli
Liquid (sterile filtered)
1 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
None
None
Dry Ice
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is six (6) months from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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