Human IgM APC
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Datasheet

Human IgM APC

009-026-007
0.25 mL
Human
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$179.00 /Per Item
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Description

Background

Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.

Application Note

Human IgM whole molecule Allophycocyanin conjugated can be utilized as a control reagent in Flow Cytometry, Immunohistochemistry, and Western Blotting. Researchers should determine optimal titers for applications that are not stated.

Purity/Specificity

Human IgM whole molecule Allophycocyanin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Human IgM whole molecule Allophycocyanin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Allophycocyanin, anti-Human IgM and anti-Human Serum. No reaction was observed against anti-Human IgG F(c).

HUMAN IgM whole molecule Allophycocyanin Conjugated - 009-026-007
HUMAN IgM APC conjugated, HUMAN IgM allophycocyanin conjugation, isotype control for flow cytometry
Human
Allophycocyanine
IgM
1-2 moles APC per mole of IgM
Native Protein
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
0.1% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Wet Ice
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable 4° C as an undiluted liquid. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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