Human Collagen Type IV
SDS-PAGE Results of Human Collagen Type IV
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Datasheet

Human Collagen Type IV

009-001-106
500 µg
SDS-PAGE, ELISA
Human
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Description

Background

Collagen type IV is a type of collagen found primarily in the basal lamina. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen type IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause collagen IV to form in a sheet, the form of the basal lamina. There are six human genes associated with collagen IV: COL4A1, COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6.

Application Note

Collagen type IV has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

Purity/Specificity

This product has been prepared from human placenta and is chromatographically and immunologically pure.  This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.  This product reacts with anti-Collagen Type IV.  Reaction with Rockland’s anti-Collagen I, II, III, V or VI is negligible (typically less than 1% cross-reactivity was detected by ELISA).

Human COLLAGEN Type IV - 009-001-106
Type IV collagen, collagen 4, human collagen, Collagen alpha-4 (IV) chain
COL4A1-COL4A6
SDS-PAGE
ELISA
Human
Protein
Native Protein
Liquid (sterile filtered)
1.0 mg/ml by modified Lowry assay
0.5 M Acetic Acid
0.01% (w/v) Sodium Azide
None
Wet Ice
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is six (6) months from date of opening.
Wang L et al. Knockdown of lncRNA NORAD inhibits the proliferation, inflammation and fibrosis of human mesangial cells under high‑glucose conditions by regulating the miR‑485/NRF1 axis. Exp Ther Med. (2021)
Applications
EIA, ELISA

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No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.; This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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