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Datasheet

Glutathione-S-Transferase (GST) Control Protein

1 References
000-001-200
100 µg
Liquid (sterile filtered)
WB, SDS-PAGE, Biochemical Assay
E. coli
$399.00 /Per Item
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Background

Glutathione-S-Transferase (GST) Control Protein will bind to glutathione affinity resin and will be detected by Anti-GST antibody. Affinity tags are appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include Glutathione-S-Transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag.

Product Details

Glutathione-S-Transferase (GST) Control Protein - 000-001-200
control protein, GST protein, GST control, Glutathione-S-Transferase control, Glutathione-S-Transferase protein
E. coli

Target Details

Glutathione-S-Transferase (GST) Control Protein was purified by chromatography from E. coli preparations. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-GST antibody. Analysis by SDS-PAGE resulted in a pattern consistent with purified GST and an estimated purity greater than 90%.

Application Details

SDS-PAGE, WB
Biochemical Assay  - View References
GST Control Protein is suitable for use as a control in affinity purification and in western blot immunoassays and has been tested in SDS-PAGE and WB. Optimal concentration should be determined by the researcher. Expect a band at ~25.5 kDa.

Formulation

1.0mg/mL by BCA assay
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is six (6) months from date of receipt.
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Applications
In Vitro Binding Assay

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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