ERK2 phosphoT185-pY187 hu recomb protein
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Datasheet

ERK2 phosphoT185-pY187 hu recomb protein

009-001-GR0
10 µg
Human, Mouse, Rat
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Description

Background

ERK2 pT185-pY187 activated recombinant protein was produced in Sf9 cells. Mitogen activated protein kinase 1, also known as MAPK1, ERK, or ERK2, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. This recombinant protein is the activated ERK1 isoform and is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Application Note

ERK2 pT185-pY187 is an activated recombinant protein suitable as a control for polyclonal or monoclonal anti-ERK in immunological assays. ERK2 can be activated by in vitro phosphorylation by MEK proteins. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Purity/Specificity

ERK2 pT185-pY187 is a recombinant protein containing a polyhistidine tag expressed in Sf9. ERK2 was phosphorylated in vitro using the activated MEK. Analysis by SDS-PAGE resulted in a pattern consistent with purified ERK2 and was estimated to be greater than 90% pure.

ERK2 pT185-pY187 human recombinant protein - 009-001-GR0
MAPK3, ERK, ERK1, PRKM3, ERK-1 Antibody, MAPK1, ERK-2, ERK2, MAPK2, P42MAPK, PRKM1, PRKM2, ERK-2 recombinant protein
MAPK1
Human, Mouse, Rat
Dual Modification
Human
Recombinant Protein
Liquid (sterile filtered)
None
Dry Ice
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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