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Datasheet

ERK2 double mutant hu recomb protein

009-001-GR2
10 µg
Liquid (sterile filtered)
Human
Sf9 cells
This product is discontinued

Background

ERK2 isoform double mutant recombinant protein is modified to put alanine residues at two key activation sites, Threonine 185 and Tyrosine 222. Mitogen activated protein kinase 1, also known as MAPK1, ERK, or ERK2, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. The inactive double mutant ERK2 recombinant protein is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Product Details

ERK2 double mutant human recombinant protein - 009-001-GR2
MAPK1, ERK, ERK-2, ERK2, MAPK2, P42MAPK, PRKM1, PRKM2, ERK-2 recombinant protein
Human
Sf9 cells
Recombinant Protein

Target Details

MAPK1  - View All MAPK1 Products
ERK2 double mutant human is a recombinant protein containing a polyhistidine tag expressed in Sf9. Analysis by SDS-PAGE resulted in a pattern consistent with purified ERK2 and was estimated to be greater than 90% pure.

Application Details

ERK2 mutant protein is suitable as a negative control protein for immunoassays using antibodies targeting the critical ERK2 phosphorylated T185 or Y187 residues. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Formulation

None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.