ERK1 double mutant hu recomb protein
SDS-PAGE results of ERK1 double mutant control protein
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Datasheet

ERK1 double mutant hu recomb protein

009-001-GR1
10 µg
SDS-PAGE
Human
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Description

Background

ERK1 isoform double mutant recombinant protein is modified to put alanine residues at two key activation sites, Threonine 202 and Tyrosine 204. Mitogen activated protein kinase 3, also known as MAPK3, ERK, or ERK1, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. The inactive double mutant ERK1 recombinant protein is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Application Note

Human ERK1 mutant recombinant protein has been tested in SDS-Page and is suitable as a negative control protein for immunoassays using antibodies targeting the critical ERK1 phosphorylated T202 or Y204 residues. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Purity/Specificity

ERK1 double mutant human is a recombinant protein containing a polyhistidine tag expressed in Sf9. Analysis by SDS-PAGE resulted in a pattern consistent with purified ERK1 and was estimated to be greater than 90% pure.

ERK1 double mutant human recombinant protein - 009-001-GR1
MAPK3, ERK, ERK1, PRKM3, ERK-1 recombinant protein
MAPK3
SDS-PAGE
Human
Sf9 cells
Recombinant Protein
Liquid (sterile filtered)
None
Dry Ice
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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