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Datasheet

MAPK3 Antibody

Rabbit Polyclonal

600-401-R20
100 µg
WB, IHC
Human, Mouse, Rat
Rabbit
$445.00 /Per Item
Availability: Ships in approximately 14 days
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Mitogen-activated protein kinase 3 is an enzyme that in humans is encoded by the MAPK3 gene. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. MAPK3 gene is mapped to human chromosome 16 by hybrid cell panel analysis. This antibody is suitable for researchers interested in stem cell, neuroscience and cancer research.

Product Details

Anti-MAPK3 (RABBIT) Antibody - 600-401-R20
ERT2,Extracellular signal-regulated kinase 1,
Rabbit
Polyclonal
IgG

Target Details

MAPK3  - View All MAPK3 Products
Human, Mouse, Rat
Conjugated Peptide
MAPK3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human MAPK3.
Anti-MAPK3 antibody is directed against human MAPK3 protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest reactivity with this protein from human, mouse, and rat based on 100% homology for the immunogen sequence.  Cross reactivity with MAPK3 from other sources has not been determined.

Application Details

IHC, WB
Anti-MAPK3 is tested for Immunohistochemistry -P, Immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~43.1 kDa corresponding to the appropriate cell lysate or extract.

Formulation

Lyophilized
0.5 mg/mL by UV absorbance at 280 nm
0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3
0.05mg Thimerosal
5mg BSA
100 µL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.