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Dog IgG
4 References
004-0102
004-0102-1000
004-0102-0005
10 mg
1 g
5 mg
Lyophilized
Liquid (sterile filtered)
Lyophilized
WB, ELISA, SDS-PAGE, Biochemical Assay, Functional Assay
Dog
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Dog IgG Whole Molecule (BULK ORDER) - 004-0102
Dog immunoglobulin G, Canine IgG
Dog
IgG
Native Protein
Target Details
Dog IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Dog IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog IgG and anti-Dog Serum.
Application Details
SDS-PAGE
Biochemical Assay, ELISA, Functional Assay, WB
- View References
Dog IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Formulation
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dog IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Walsh S et al. (2025). Structural and functional characterization of IdeC, a novel IgG-specific protease of Streptococcus canis. Infect Immun.
Applications
EIA, ELISA; SDS, 2D-PAGE; WB, IB, PCA; SPR-Based Analyses
Olivry T et al. (2024). Validation of a Multiplex Molecular Macroarray for the Determination of Allergen-Specific IgE Sensitizations in Dogs. Vet Sci.
Applications
EIA, ELISA
Sewid AH et al. (2018). Identification, Cloning, and Characterization of Staphylococcus pseudintermedius Coagulase. Infect Immun.
Applications
In Vitro Binding Assay
Abouelkhair, MA et al. (2018). Characterization of recombinant wild-type and nontoxigenic protein A from Staphylococcus pseudintermedius. Virulence
Applications
EIA, ELISA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
