Chicken IgG Fluorescein
SDS-PAGE of Chicken IgG/IgY Whole Molecule Fluorescein Conjugated (p/n 003-0202)
Datasheet is currently unavailable. Try again or CONTACT US
Datasheet

Chicken IgG Fluorescein

003-0202
1 mg
SDS-PAGE
Have a Question? Ask a Specialist
$108.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Application Note

CHICKEN IgG whole molecule Fluorescein conjugated has been tested by SDS-PAGE and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purity/Specificity

This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by extensive dialysis against the buffer stated above.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Chicken IgG and anti-Chicken Serum.

CHICKEN IgG whole molecule Fluorescein conjugated - 003-0202
Chicken IgG fluorescein conjugated, Chicken IgG FITC conjugated, Chicken IgY fluorescein conjugated, Chicken IgY FITC conjugated
SDS-PAGE
Chicken
Fluorescein (FITC)
IgG
3.5 moles FITC per mole of IgG
Protein
Native Protein
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.

Invalid lot number

If you need help finding your CofA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.