Chicken IgG Fab
SDS-PAGE of Chicken IgG/IgY Fab Fragment
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Datasheet

Chicken IgG Fab

003-0105 003-0105-0002
5 mg 2 mg
SDS-PAGE, Cellular Assay, Functional Assay
Chicken
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Description

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.

Application Note

Chicken IgG F(ab) has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Purity/Specificity

This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken IgG, anti-Chicken IgG F(ab’)2 and anti-Chicken Serum.  No reaction was observed against anti-Chicken IgG F(c) or anti- Papain.

CHICKEN IgG Fab fragment (BULK ORDER) - 003-0105
Chicken IgG Fab fragment, Chicken IgY Fab fragment
SDS-PAGE
Cellular Assay, Functional Assay
Chicken
IgG Fab
Protein
Native Protein
Liquid (sterile filtered)
5.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Wet Ice
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of opening.
Moore RW et al. Effect of bursal anti-steroidogenic peptide and immunoglobulin G on neonatal chicken B-lymphocyte proliferation. Comp Biochem Physiol C Toxicol Pharmacol. (2003)
Applications
Other

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.