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CDKL1 protein-GST fusion
$50.00 to US & Canada for most products. Final costs are calculated at checkout.
Description
Background
CDKL1 or cyclin-dependent kinase-like 1 is a member of a large family of CDC2-related serine/threonine protein kinases. CDKL1, which is also known as p42 KKIALRE, accumulates primarily in the nucleus and is known to regulate important transitions in the eukaryotic cell cycle (1). CDKL1 and the related CDC2 protein kinase have a similar pattern of mRNA expression except in the reproductive organs. CDKL1 is expressed in several human tissues, with the highest expression in kidney and ovary (2). CDKL1 is overexpressed in breast cancer patients and has a positive detection efficiency of 77%. Inhibiting CDKL1 function with shRNA in MCF-7 cells shows increased accumulation of the cells at the G2/M phase and increased sensitivity to cell cycle chemotherapeutic drugs. CDKL1 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cancer, Cell Cycle, and Ser/Thr Kinases research.
Application Note
CDKL1 Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. CDKL1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 60kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Purity/Specificity
Recombinant full-length human CDKL1 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.