BUB1 protein-GST fusion
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Datasheet

BUB1 protein-GST fusion

009-001-R54S 009-001-R54
20 µg 50 µg
Human
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$309.00 /Per Item
$629.00 /Per Item
20 µg $309.00
50 µg $629.00
Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days
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Description

Background

BUB1 is a kinase which involved in spindle checkpoint function that in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. BUB1 is highly expressed in thymus, ovary, and testis (1). BUB1 maintains the steady-state level and centromeric localization of SGO1, and they concluded that BUB1 protects centromeric cohesion in mitosis through SGO1 (2). BUB1-mediated phosphorylation of CDC20 is required for proper checkpoint signaling and that inhibition of APC by BUB1 may partly account for the sensitivity of the spindle checkpoint. BUB1 kinase creates a mark for shugoshin localization and the correct partitioning of chromosomes. BUB1 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cancer, Cell Cycle, and Ser/Thr Kinases research.

Application Note

BUB1 Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. BUB1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 63kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Purity/Specificity

Recombinant human BUB1 (756-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.

BUB1 recombinant protein-GST fusion protein - 009-001-R54
BUB1A, BUB1L, hBUB1, Mitotic checkpoint serine/threonine-protein kinase BUB1
BUB1
Human
Sf9 cells
Recombinant Protein
Liquid (sterile filtered)
0.1 µg/µL
See application note.
Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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