BCR protein-GST fusion
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Datasheet

BCR protein-GST fusion

009-001-R50S 009-001-R50
20 µg 50 µg
Human
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$298.00 /Per Item
$579.00 /Per Item
20 µg $298.00
50 µg $579.00
Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

BCR (also known as breakpoint cluster region) encodes a phosphoprotein that has serine/threonine kinase activity (1). The human c-ABL oncogene is translocated from chromosome 9 to a region on chromosome 22 (associated with BCR) in chronic myelocytic leukemia. In classical t(9;22) translocation, as observed in chronic granulocytic leukemia (CGL), a hybrid DNA unit is produced comprising the BCR gene product plus the translocated c-ABL gene from chromosome 9. The BCR-ABL hybrid protein (p210) is formed that displays increased tyrosine kinase activity. A similar DNA rearrangement of the p210 protein is also found in cases of Philadelphia-positive acute lymphoblastic leukemia (ALL) (2). BCR Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, and Cancer research.

Application Note

BCLR Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. BCLR Protein is suitable for use in Western Blot. Expect a band approximately ~ 120kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Purity/Specificity

Recombinant human BCR (1-695) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.

BCR recombinant protein-GST fusion protein - 009-001-R50
ALL, CML, PHL, BCR1, D22S11, D22S662, FLJ1645, Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26, BCR, BCR1, D22S11
BCR
Human
Sf9 cells
Protein
Recombinant Protein
Liquid (sterile filtered)
0.2 µg/µL
See application note.
Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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