ATG13 protein-GST fusion
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Datasheet

ATG13 protein-GST fusion

009-001-R44S 009-001-R44
20 µg 50 µg
Human
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$298.00 /Per Item
$579.00 /Per Item
20 µg $298.00
50 µg $579.00
Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

ATG13 is a protein that is part of the autophagy complex which is the major route by which cytoplasmic contents are delivered to the lysosome for degradation. ATG13 localizes on the autophagic isolation membrane and is essential for autophagosome formation. In mammals, ATG13 interacts with ATG1 (ULK1/2) and FIP200 to form the autophagy. ATG101 can also bind to ATG13 protein and is important for the stability and basal phosphorylation of ATG13 and ULK1 (1). mTOR can suppress the autophagy process through direct regulation of the ULK1-Atg13-FIP200 complex where mTOR has been shown to phosphorylate ATG13 (2). ATG13 Protein is ideal for investigators involved in Signaling Proteins, Apoptosis Proteins, Angiogenesis, Cardiovascular Disease, Cellular Stress, Inflammation, Metabolic Disorder, Neurobiology, NfkB Pathway, and p38 Pathway research.

Application Note

ATG13 Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. ATG13 Protein is suitable for use in Western Blot. Expect a band approximately ~ 96kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Purity/Specificity

Recombinant full-length human ATG13 was expressed by baculovirus in E.coli using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

ATG13 recombinant protein-GST fusion protein - 009-001-R44
KIAA0652, Autophagy-related protein 13, ATG14
ATG13
Human
E. coli
Protein
Recombinant Protein
Liquid (sterile filtered)
0.1 µg/µL
See application note.
25% (v/v) Glycerol
Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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