Tryptophan Hydroxylase phospho S260 Antibody
Western blot - Tryptophan Hydroxylase phospho S260 Antibody
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Datasheet

Tryptophan Hydroxylase phospho S260 Antibody

Rabbit Polyclonal

600-401-E49
100 µL
WB
Human, Rat
Rabbit
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Description

Background

Tryptophan Hydroxylase pS260 antibody detects Tryptophan Hydroxylase, which catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH. Anti-THPpS260 Antibody is ideal for investigators involved in Cell Signaling, and Neuroscience research.

Application Note

Anti-Tryptophan Hydroxylase pS260 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of 55 kDa in size corresponding to typtophan hydroxylase protein phosphorylated at Ser260 in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Purity/Specificity

Anti-Tryptophan Hydroxylase pS260 antibody is directed against rat Tryptophan Hydroxylase. Tryptophan Hydroxylase pS260 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity is expected from the following species based on 100% sequence homology: bovine, canine, chicken, mouse and zebra fish.

Anti-Tryptophan Hydroxylase pS260 (Rabbit) Antibody - 600-401-E49
Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1, TPH
Tph1
WB
Rat
Human, Rat
Phosphorylation
Rabbit
Polyclonal
IgG
Peptide
Anti-Tryptophan Hydroxylase pS260 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 of Tryptophan Hydroxylase.
Liquid
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Dry Ice
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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