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Troponin I (cardiac) Phospho S150 Antibody

Rabbit Polyclonal

100 µL
Mouse, Rat
$475.00 /Per Item
Availability: Ships in approximately 14 days
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Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 and is phosphorylated by AMPK at Ser23 and Ser150. Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser150 over Ser23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI. Troponin I pS150 Antibody is ideal for researchers interested in cancer research.

Product Details

Anti-Troponin I (cardiac) pS150 (RABBIT) Antibody - 600-401-X33
Troponin I cardiac muscle, Cardiac troponin I

Target Details

Tnni3  - View All Tnni3 Products
Mouse, Rat
Conjugated Peptide
Anti-Phospho-Ser150 Troponin (cardiac) Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 pf cardiac troponin I conjugated to KLH.
Anti-Phospho-Ser150 Troponin (cardiac) Antibody is directed against Troponin protein phosphorylated at S150. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated Troponin. Minimal reactivity occurs against non-phosphorylated Troponin. Reactivity against Troponin occurs from mouse sources. However, reactivity is also expected against human and non-human primate based on 100% sequence homology.

Application Details

Anti-Phospho-Ser150 Troponin (cardiac) Antibody has been tested in Western Blots and is specific for the ~25 kDa cardiac troponin I protein phosphorylated at Ser 150. Immunolabeling is greatly decreased with lambda-phosphatase treatment. Specific conditions for reactivity should be optimized by the end user.


Liquid (sterile filtered)
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Shipping & Handling

Wet Ice
Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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