TNF alpha Antibody

Description

Background

Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNF is an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD). Anti-TNF alpha Antibody is useful for researches interested in TNF/TNFR pathways, apoptosis signaling pathways, and cell proliferation.

Synonyms

APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody, Tumour Necrosis Factor Alpha antibody, rabbit anti-Tumor Necrosis Factor Alpha Antibody, rabbit anti-TNF Alpha Antibody, Tumor necrosis factor, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2

Immunogen

TNF alpha antibody used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFα.

Application Note

Anti-TNF alpha antibody has been tested in WB, ELISA, IF, and IHC. Expect a band in appropriate lysates at ~25.6kDa. This antibody is also useful for neutralization of human and primate TNFα activity in bioassays diluted at 1:200. The antibody does not recognize human TNF beta (lymphotoxin). Specific conditions for reactivity and signal detection should be optimized by the end user.

Purity/Specificity

This antibody is primarily directed against mature 17,000 MW human TNFα and is useful in determining its presence in various assays.  In general, this antibody also detects primate TNFα in the same formats using similar dilutions.  The antibody does not recognize human TNFß (lymphotoxin).   This IgG fraction antibody will recognize the cell-bound precursor of TNFα as a 26,000 protein in immunoblots, particularly in denatured samples.  This antibody is also useful for neutralization of human and primate TNFα activity in bioassays.  It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG is recommended.

Storage Condition

Store anti-TNF alpha at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.