SPANX-C Antibody
Anti-SPANX-C Antibody - Immunohistochemistry
Anti-SPANX-C Antibody - Western Blot
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Datasheet

SPANX-C Antibody

Rabbit Polyclonal

200-401-915
500 µg
IHC, WB
Human
Rabbit
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$456.00 /Per Item
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Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Human Sperm Proteins Associated with the Nucleus on X-chromosome (SPANX) are relatively low molecular weight cytoplasmic proteins found in testis and sperm.  Their expression in other tissues indicates malignancies. Family members are observed as proteins that range from 15 to 20 kDa.

Application Note

This protein A purified antibody has been tested for use in ELISA, immunohistochemistry, and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15-20 kDa in size corresponding to SPANX family members by western blotting in the appropriate human tissue.  Most human cell lines are SPANX negative.

Purity/Specificity

This Protein A purified antibody is directed against human SPANX-C protein.  The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.   A protein-protein BLAST analysis was used to suggest cross reactivity with most SPANX proteins from human, gorilla and chimpanzee.  Reactivity against homologues from is not known.

Anti-SPANX-C (RABBIT) Antibody - 200-401-915
rabbit anti-SPANX-C antibody, Cancer-testis-associated protein CTp11 antibody, CTP11 antibody, Nuclear associated protein SPAN-Xc antibody, SPANX family member C antibody, Sperm protein associated with the nucleus on the X chromosome C, SPANX family member C1 antibody, SPANXC
SPANXC
IHC, WB
Human
Human
Rabbit
Polyclonal
IgG
Recombinant Protein
This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant human SPANX-C protein.
Lyophilized
5.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
100 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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