SOX2 Antibody
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Datasheet

SOX2 Antibody

Rabbit Polyclonal

600-401-ES5
100 µg
ELISA, IF, IHC, WB
Human, Mouse, Rat
Rabbit
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Description

Background

SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. SOX2 is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. The role of SOX2 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing SOX2 and the transcription factors POU5F1, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Other experiments have shown that iPS cells could be generated using expression plasmids expressing POU5F1, SOX2, KlfF4 and c-Myc, eliminating the need for virus introduction.

Application Note

Anti-SOX2 Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry, Immunohistochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 34 kDa in Western Blots of specific cell lysates and tissues.

Purity/Specificity

Anti-SOX2 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with SOX2 from other sources has not been determined.

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-SOX2 (RABBIT) Antibody - 600-401-ES5
SOX2 Antibody, ANOP3, MCOPS3, Transcription factor SOX-2
SOX2
ELISA, IF, IHC, WB
Human
Human, Mouse, Rat
Rabbit
Polyclonal
IgG
Peptide
Anti-SOX2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide near the N-terminus of human SOX.
Liquid (sterile filtered)
Dry Ice
Expiration date is one (1) year from date of opening.
1 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.