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Rho Antibody
Mouse Monoclonal 4D2 IgG1
200-301-G36
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC, IF, IP
Rat, Acanthodes bridgei, Amphibians, Avian, Fish, Mammalian
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Rho (MOUSE) Monoclonal Antibody - 200-301-G36
OPN2 Antibody, opsd Antibody, opsin 2 Antibody, opsin 2 rod pigment Antibody, opsin2 Antibody, RHO Antibody, RP4 Antibody, MGC138309 Antibody, Retinitis Pigmentosa 4 Antibody, Rhodopsin Antibody
Mouse
Monoclonal
IgG1
Target Details
RHO - View All RHO Products
Rat, Acanthodes bridgei, Amphibians, Avian, Fish, Mammalian
Other
Rho Antibody was produced in mice by repeated immunizations raised against bovine Rhodopsin.
Anti-Rho Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Rhodopsin from Mammals, Fish, Avian, Amphibians, and Shark based on 100% homology with the immunizing sequence. Not Drosophila or other invertebrates. Cross-reactivity with Rhodopsin from other sources has not been determined. Signaling and GTPase research.
P02699 - UniProtKB
NP_001014890.1 - NCBI Protein
Application Details
ELISA, IF, IHC, IP, WB
Anti-Rho Antibody is recommended for use in IF microscopy, IHC, IP, WB, and ELISA. Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Specific conditions for reactivity should be optimized by the end user.
Formulation
1.0 mg/ml by UV absorbance at 280 nm
1X PBS, pH 7.4
0.09% (w/v) Sodium Azide
50% (v/v) Glycerol
Shipping & Handling
Wet Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket. In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chromophore to the surface of the molecule upon light absorption. In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
