RFP Antibody
Western Blot of Goat Anti-RFP Antibody
Immunohistochemistry of Goat Anti-RFP
Immunohistochemistry of Goat Anti-RFP
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Datasheet

RFP Antibody

Goat Polyclonal

200-101-379S 200-101-379
25 µL 100 µg
SDS-PAGE, WB, EM, FC, IF, IHC
RFP, rRFP
Goat
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$125.00 /Per Item
$449.00 /Per Item
25 µL $125.00
100 µg $449.00
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Description

Background

Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS).

Application Note

Polyclonal anti-RFP has been tested by SDS-Page and western blot and is designed to detect RFP and its variants. Optimal titers for applications should be determined by the researcher. Expect a band ~27kDa in Western Blot in the appropriate cell lysate or extract.

Purity/Specificity

Anti-RFP (GOAT) is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins.   Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

Anti-RFP (GOAT) Antibody - 200-101-379
goat anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
DsRed
SDS-PAGE, WB
EM, FC, IF, IHC
RFP, rRFP
Goat
RFP
Polyclonal
IgG
Recombinant Protein
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Liquid (sterile filtered)
1 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Generation of a CRF1-Cre transgenic rat and the role of central amygdala CRF1 cells in nociception and anxiety-like behavior. ELife ()
Applications
IF, Confocal Microscopy
Edinger-Westphal peptidergic neurons enable maternal preparatory nesting. Neuron ()
Applications
IHC, ICC, Histology
Saccharin Stimulates Insulin Secretion Dependent on Sweet Taste Receptor-Induced Activation of PLC Signaling Axis. Biomedicines ()
Applications
IF, Confocal Microscopy
Saccharin Stimulates Insulin Secretion Dependent on Sweet Taste Receptor-Induced Activation of PLC Signaling Axis. Biomedicines. ()
Applications
IF, Confocal Microscopy
Inhibitory Fear Memory Engram in the Mouse Central Lateral Amygdala. Cell Press Preprint ()
Applications
Undefined
Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacity. Genes Cells. ()
Applications
IF, Confocal Microscopy
Wnt-modified materials mediate asymmetric stem cell division to direct human osteogenic tissue formation for bone repair. Nat Mater. ()
Applications
Undefined
Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells. J Cell Biol. ()
Applications
IF, Confocal Microscopy
Targeting KDM4A epigenetically activates tumor-cell-intrinsic immunity by inducing DNA replication stress. Mol Cell. ()
Applications
Undefined
mGlu1 potentiation enhances prelimbic somatostatin interneuron activity to rescue schizophrenia-like physiological and cognitive deficits. Cell Rep. ()
Applications
IF, Confocal Microscopy
Wolframin-1–expressing neurons in the entorhinal cortex propagate tau to CA1 neurons and impair hippocampal memory in mice. Sci Transl Med. ()
Applications
IHC, ICC, Histology; Immuno-EM
Generation and validation of a CRF1:Cre transgenic rat: The role of central amygdala CRF1 in nociception and anxiety-like behavior. bioRxiv Preprint ()
Applications
IF, Confocal Microscopy
Neuroblastoma differentiation in vivo excludes cranial tumors. Dev Cell. ()
Applications
Undefined
Fibrosis Without Myofibroblasts Revealed by Genetic Analysis of PDGFRα. Cell Press Preprint ()
Applications
Undefined
Astrocytes regulate ultra-slow arteriole oscillations via stretch-mediated TRPV4-COX-1 feedback. Cell Rep. ()
Applications
IHC, ICC, Histology
Origin, specification and differentiation of a rare supporting-like lineage in the developing mouse gonad. HAL Preprint. ()
Applications
IHC, ICC, Histology
Spatiotemporal dynamics of PIEZO1 localization controls keratinocyte migration during wound healing. Elife. ()
Applications
IF, Confocal Microscopy
In vitro reconstitution of the whole male germ-cell development from mouse pluripotent stem cells. Cell Stem Cell. ()
Applications
Undefined
A Specific Neuroligin3-αNeurexin1 Code Regulates GABAergic Synaptic Function in Mouse Hippocampus. ELife ()
Applications
IF, Confocal Microscopy
Krüppel-like Factor 5 Regulates Stemness, Lineage Specification, and Regeneration of Intestinal Epithelial Stem Cells. Cell Mol Gastroenterol Hepatol. ()
Applications
IF, Confocal Microscopy; FC, FACS, FLOW
Stromal CCL2 Signaling Promotes Mammary Tumor Fibrosis through Recruitment of Myeloid-Lineage Cells. Cancers (Basel). ()
Applications
IF, Confocal Microscopy
Vasoactive Intestinal Polypeptide-Expressing Interneurons in the Hippocampus Support Goal-Oriented Spatial Learning. Neuron. ()
Applications
IHC, ICC, Histology
A Mac2-positive progenitor-like microglial population survives independent of CSF1R signaling in adult mouse brain. Elife. ()
Applications
IHC, ICC, Histology
Acute inflammation regulates neuroregeneration through the NF-κB pathway in olfactory epithelium. Proc Natl Acad Sci U S A. ()
Applications
IHC, ICC, Histology
Comparative Genomic Analysis of Drechmeria coniospora Reveals Core and Specific Genetic Requirements for Fungal Endoparasitism of Nematodes. PloS Genetics ()
Applications
WB, IB, PCA
Regulated delivery of molecular cargo to invasive tumour-derived microvesicles. Nature Communications ()
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.