Potassium Chloride Cotransporter (KCC2) phospho S940 Antibody
Western blot of Anti-Potassium Chloride Cotransporter (KCC2) pS940 (Rabbit) Antibody - 612-401-E15
Datasheet is currently unavailable. Try again or CONTACT US
Datasheet SDS

Potassium Chloride Cotransporter (KCC2) phospho S940 Antibody

Rabbit Polyclonal

5 References
612-401-E15
100 µL
WB, IP
Rat
Rabbit
$475.00 /Per Item
Request a Quote
Bulk Orders
Availability: Ships in approximately 14 days
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Potassium Chloride Cotransporter pS940 Antibody detects Potassium Chloride Cotransporter pS940 protein. KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser940 increased the cell surface stability of KCC2 in this system by decreasing it’s rate of internalization from the plasma membrane. Anti-Potassium Chloride Cotransporter (KCC2) pS940 Antibody is ideal for investigators involved in Neuroscience and Cell Signaling research.

Product Details

Anti-Potassium Chloride Cotransporter (KCC2) pS940 (RABBIT) Antibody - 612-401-E15
Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, Furosemide-sensitive K-Cl cotransporter, K-Cl cotransporter 2, rKCC2, Neuronal K-Cl cotransporter, Kcc2
Rabbit
Polyclonal
IgG

Target Details

Slc12a5  - View All Slc12a5 Products
Rat
Phosphorylation
Conjugated Peptide
Anti-Potassium Chloride Cotransporter (KCC2) pS940 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940.
Anti-Potassium Chloride Cotransporter (KCC2) pS940 antibody is directed against phosphorylated rat KCC2 protein. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Immunolabeling of the KCC2 protein band is blocked by the phospho-peptide used as antigen but not by the corresponding dephosphopeptide. Reactivity is expected from the following species based on 100% sequence homology: bovine, canine, human, mouse and non-human primate.
UniProtKB - Q63633
GeneID - 171373
UniProtKB - Q63633.2

Application Details

IP, WB
Anti-Potassium Chloride Cotransporter (KCC2) Antibody is tested for use in IP and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 135 kDa in size corresponding to KCC2 protein phosphorylated at Ser940 in the appropriate cell lysate or extract.

Formulation

Liquid
Sufficient for 10 mini blots (total final volume 100 ml at 1:1000 dilution) by UV absorbance at 280 nm
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
None
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). Brain-Derived Neurotrophic Factor and Supraoptic Vasopressin Neurons in Hyponatremia. Neuroendocrinology.
Applications
WB, IB, PCA
(). A cellular mechanism of learning-induced enhancement of synaptic inhibition: PKC-dependent upregulation of KCC2 activation. Sci Rep.
Applications
WB, IB, PCA
(). Quantitative phosphoproteomic analysis of the molecular substrates of sleep need. Nature.
Applications
WB, IB, PCA
(). High salt loading increases brain derived neurotrophic factor in supraoptic vasopressin neurones. J Neuroendocrinol.
Applications
WB, IB, PCA
(). AT1a influences GABAA-mediated inhibition through regulation of KCC2 expression. Am J Physiol Regul Integr Comp Physiol.
Applications
WB, IB, PCA

Invalid lot number

If you need help finding your CoA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Related Resources

GFP Antibodies: A Comprehensive Guide

Articles
View Article

Custom Recombinant Antibodies (rAbs)

Learn More

Custom Antibody Production

Learn More