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Datasheet

NOTCH 1 Antibody

Rabbit Polyclonal

1 References
100-401-405
200 µL
Liquid (sterile filtered)
ELISA, IHC
Human
Rabbit
$475.00 /Per Item
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Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Notch-1 is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Anti-Notch 1 Antibody is useful for researchers interested in Notch pathways, cancer research, transcription factors, and DNA binding research.

Product Details

Anti-NOTCH 1 (intra) (RABBIT) Antibody - 100-401-405
rabbit anti-notch1 antibody, hN1 antibody, Neurogenic locus Notch homolog protein 1 antibody, Notch 1 intracellular domain antibody, Notch homolog 1 translocation associated antibody, TAN1 antibody, Translocation associated Notch protein TAN1 antibody
Rabbit
Polyclonal
Antiserum

Target Details

NOTCH1  - View All NOTCH1 Products
Human
Conjugated Peptide
Anti-Notch antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid 2480-2510 of human Notch 1.  A residue of cysteine was added to the amino terminal end to facilitate coupling.
Notch1 antibody is directed against human NOTCH 1.  No reaction is detected against NOTCH 2.  No reactivity was observed against Mouse Notch.  Other species have not been tested.

Application Details

ELISA, IHC
Anti-Notch-1 has been tested in IHC and ELISA. This antibody is useful in western blot. Specific conditions for reactivity should be optimized by the end user.

Formulation

75 mg/mL by Refractometry
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). ErbB2 induces Notch1 activity and function in breast cancer cells. Clinical and Translational Science
Applications
IHC, ICC, Histology

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If you need help finding your CoA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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