NMDA R1, Splice Variant C2' Antibody
Western blot of Anti-NMDA R1, Splice Variant C2' (Rabbit) Antibody - 600-401-D87
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Datasheet

NMDA R1, Splice Variant C2' Antibody

Rabbit Polyclonal

600-401-D87
25 µg
IHC, WB
Mouse, Rat
Rabbit
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Description

Background

NMDA R1, Splice Variant C2' Antibody detects NMDA R1 protein. The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death. Increased membrane surface expression of the NMDAR, NR1-Subunit has been associated with synaptic plasticity. There are a number of different splice variants of the NR1-Subunit. Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR. Anti-NMDA R1, Splice Variant C2' Antibody is ideal for investigators involved in Neuroscience and Signal Transduction research.

Application Note

Anti-NMDA R1, Splice Variant C2' (Rabbit) Antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 120 kDa in size corresponding to the NMDA receptor containing the C2' splice variant insert in the appropriate cell lysate or extract.

Purity/Specificity

Anti-NMDA R1, Splice Variant C2' antibody is directed against NMDA R1 protein. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. This antibody does not recognize the NR1 subunits of the NMDA receptor that do not contain the C2’ insert. Reactivity is expected from the following species based on 100% sequence homology: bovine, human.

Anti-NMDA R1, Splice Variant C2' (Rabbit) Antibody - 600-401-D87
Glutamate [NMDA] receptor subunit zeta-1,N-methyl-D-aspartate receptor subunit NR1, NMD-R1, Grin1
Grin1
IHC, WB
Rat
Mouse, Rat
Rabbit
Polyclonal
IgG
Peptide
Anti-NMDA R1, Splice Variant C2' Antibody was produced by repeated immunizations with synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, C2' splice variant insert.
Lyophilized
50µL
Neutral PBS
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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