Datasheet is currently unavailable. Try again or CONTACT US
Datasheet

Neurofilament M Antibody

Chicken Polyclonal

4 References
212-901-D84
100 µL
WB, IHC, IF
Human, Mouse, Rat, Bovine, Chicken
Chicken
$475.00 /Per Item
Availability: Ships in approximately 14 days
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

NF-M antibody detects Neurofilament M protein. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Neurofilament M Antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.

Product Details

Anti-Neurofilament M (Chicken) Antibody - 212-901-D84
chicken anti-Neurofilament M antibody, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein
Chicken
Polyclonal
IgY

Target Details

Nefm  - View All Nefm Products
Human, Mouse, Rat, Bovine, Chicken
Recombinant Protein
Anti-NF-M Monoclonal Antibody was produced by repeated immunizations with recombinant C-terminus part of NF-M expressed in E. coli.
Anti-NF-M antibody is directed against NF-M protein. The antibody is a total IgY fraction. Expect reactivity with the following species based on sequence homology: chicken, human, mouse. Cross reactivity with NF-M from other species has not been determined.

Application Details

IF, WB
IHC  - View References
Anti- Neurofilament M antibody is tested for use in Western Blotting and IF. Specific . Expect a band of approximately 145 kDa in size corresponding to NF-M proteins in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Formulation

Liquid
Titrated value sufficient to run approximately 10 mini blots.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). Properties of Glial Cell at the Neuromuscular Junction are Incompatible with synaptic repair in the SOD1G37R ALS mouse model. J Neurosci.
Applications
IHC, ICC, Histology
(). Purinergic-Dependent glial regulation of synaptic plasticity of competing terminals and synapse elimination at the neuromuscular junction. Cell Rep.
Applications
IHC, ICC, Histology
(). Dynamic neuromuscular remodeling precedes motor-unit loss in a mouse model of ALS. Elife
Applications
IHC, ICC, Histology
(). Glial cells decipher synaptic competition at the mammalian neuromuscular junction. J Neurosci.
Applications
IHC, ICC, Histology

Invalid lot number

If you need help finding your CoA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Related products to: Neurofilament M Antibody