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Datasheet

NEK2 Antibody

Rabbit Polyclonal

600-401-452
100 µg
WB, ELISA
Mouse
Rabbit
$475.00 /Per Item
Availability: Ships next business day
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$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G2-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins.

Product Details

Anti-NEK2 (RABBIT) Antibody - 600-401-452
rabbit anti-NEK2 antibody, Serine threonine protein kinase Nek2 antibody, HSPK 21 antibody, NEK 2 antibody, NEK2A antibody, NIMA, never in mitosis a-related kinase 2 antibody, NimA-like protein kinase 1 antibody, NIMA related kinase 2 antibody, NEK-2, NLK1, NLK-1, NEK2A, HSPK21
Rabbit
Polyclonal
IgG

Target Details

NEK2  - View All NEK2 Products
Mouse
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human NEK2 protein.
This product is an affinity-purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.  BLAST analysis indicates 100 % homology of the immunizing sequence with NEK2 homologues from human and chimpanzee.  Cross reactivity with NEK2 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.

Application Details

ELISA, WB
This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to NEK2 protein by western blotting in the appropriate cell lysate or extract.  Splice variants exist for this protein that may result in the detection of lower molecular weight bands.

Formulation

Liquid (sterile filtered)
1.1 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.