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Datasheet

Myeloperoxidase Antibody

Rabbit Polyclonal

200-4181
500 µg
Lyophilized
WB
Human
Rabbit
$525.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa).  Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle.  Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE.  Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform.  The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5%. MPO is inhibited by azide and other compounds.  MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant.  HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H2O2).  In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.

Product Details

Anti-Myeloperoxidase [Human Leukocytes] (RABBIT) Antibody - 200-4181
rabbit anti-Myeloperoxidase Antibody, rabbit anti-MPO antibody
Rabbit
Polyclonal
IgG

Target Details

MPO  - View All MPO Products
Human
Native Protein
Myeloperoxidase [Human Leukocytes]
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Myeloperoxidase [Human Leukocytes].  Cross reactivity against Myeloperoxidase from other tissues and species may occur but have not been specifically determined.

Application Details

WB
This antibody is tested by western blotting suitable for ELISA and immunoprecipitation.  Although not tested, this antibody is likely functional in immunohistochemistry and other immunological methods.  Anti-Human Myeloperoxidase may react with MPO from other sources. Anti-Human Myeloperoxidase detects neutrophilic granulocytes and monocytes in blood and precursors of granulocytes in the bone marrow. This antibody may also detect myeloid leukemias of the bone marrow as well as other sites.

Formulation

5.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
None
100 µL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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