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Datasheet

Myelin Basic Protein (MBP) Antibody

Chicken Polyclonal

200-901-D81
100 µL
WB, IF
Human, Mouse, Rat, Bovine
Chicken
$475.00 /Per Item
Availability: Ships in approximately 14 days
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Myelin Basic Protein antibody detects MBP. MBP is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells. In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22kDa and another at about 18kDa. Anti-MBP antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.

Product Details

Anti-Myelin Basic Protein (MBP) (Chicken) Antibody - 200-901-D81
Myelin A1 protein, Myelin membrane encephalitogenic protein, Myelin basic protein, MBP, Myelin basic protein S, MBP S antibody
Chicken
Polyclonal
IgY

Target Details

Mbp  - View All Mbp Products
Human, Mouse, Rat, Bovine
Conjugated Peptide
Anti-Myelin basic protein Antibody was produced in chicken by repeated immunizations with three peptide sequences conserved in higher vertebrate MBP proteins.
Anti-MBP antibody is directed against Myelin basic protein. The antibody is a total IgY fraction. The antibody has been directly tested for reactivity in bovine, human, mouse and rat tissues. Cross reactivity with MBP from other mammalian tissues is expected.

Application Details

IF, WB
Anti-Myelin Basic Protein antibody is tested for use in Western Blotting and IF. Expect a bands of approximately 18 kDa and 22 kDa in size corresponding to MBP proteins in Western blots of rat brain homogenate. Specific conditions for reactivity should be optimized by the end user.

Formulation

Liquid
Titrated value sufficient to run approximately 10 mini blots.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.