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Datasheet

Mouse TNF alpha Antibody

Rabbit Polyclonal

110-401-321S 110-401-321-0100 110-401-321
25 µL 100 µL 1 mL
WB
Mouse
Rabbit
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$99.00 /Per Item
$399.00 /Per Item
$1,102.00 /Per Item
25 µL $99.00
100 µL $399.00
1 mL $1,102.00
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Description

Background

TNF alpha Antibody detects TNF-a protein. TNF-a is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is a potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Anti-TNF-alpha Antibody is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.

Synonyms

rabbit anti-Mouse TNF alpha Antibody, APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody

Immunogen

The whole rabbit serum was prepared by repeated immunizations with recombinant mouse TNFa produced in E.coli.

Application Note

This antiserum against Mouse TNFa has been tested for use in neutralizations, ELISA, radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting.  It recognizes the 17,000 MW TNFa.  Reactivity in other immunoassays is unknown.

Purity/Specificity

This antiserum has been heated to 56°C for 30 minutes.  The antiserum is directed against mature 17,000 MW mouse TNFa and is useful in determining its presence in various assays. The antibody does not recognize mouse TNFb (lymphotoxin).   This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples.  This antibody is also useful for neutralization of mouse activity in bioassays.  It does not neutralize the biological activity of lymphotoxin.  For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG is recommended.

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Anti-Mouse TNFα (RABBIT) Antibody - 110-401-321
Tnf
WB
Mouse
Mouse
Rabbit
Unconjugated
Polyclonal
Antiserum
Recombinant Protein
Liquid (sterile filtered)
Dry Ice
Expiration date is one (1) year from date of opening.
70 mg/mL by Refractometry
None
None
None

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