Mer2 phospho S30 Antibody
Anti-Mer2 pS30 Antibody - Western Blot
Anti-Mer2 pS30 Antibody - Western Blot
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Datasheet

Mer2 phospho S30 Antibody

Rabbit Polyclonal

600-401-924
100 µg
ELISA, WB
S. cerevisiae
Rabbit
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$399.00 /Per Item
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Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Mer2 (also known as meiotic recombination 2 protein) is a chromosomal protein that is critical for meiotic recombination and progression.  It is phosphorylated at two serine residues, S30 and S271 by the yeast Cdk1 cyclin- dependent kinase homolog.  This phosphorylation is S-phase specific, and thus has the potential to be a specific assay for S-phase cyclin-dependent kinases.   Moreover, there are hints that the phosphorylation may be a mark of replication fork passage, which would indicate that S-phase CDK associates with the replication fork.

Application Note

This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to phosphorylated Mer2 protein by western blotting in the appropriate cell lysate or extract.  Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for Mer2 phosphorylated at the pS30 residue.  Preparation of extracts from cells 4hr after initiation of meiosis is suggested.

Purity/Specificity

This affinity-purified antibody is directed against the phosphorylated form of Saccharomyces cerevisiae Mer2 protein at the pS30 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated peptide to remove any unwanted reactivities.

Anti-Mer2 pS30 (RABBIT) Antibody - 600-401-924
rabbit anti-Mer2 pS30 antibody, Meiotic recombination 2 protein, Recombination protein 107, REC107, YJR021C, J1462
MER2, REC107
ELISA, WB
Saccharomyces cerevisiae
S. cerevisiae
Phosphorylation
Rabbit
Polyclonal
IgG
Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.
Liquid (sterile filtered)
1.0mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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