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Datasheet

MEK1 phospho T386 Antibody

Rabbit Polyclonal

600-401-D73
100 µL
Liquid
WB
Human, Rat
Rabbit
$475.00 /Per Item
Availability: Ships in approximately 14 days
Shipping info:

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Background

MEK1 pT386 antibody is directed against human MEK 1 phosphorylated against Thr386. MEK also known as MAP Kinase Kinase and MKK, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386. MEK1 pT386 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Product Details

Anti-MEK1 pT386 (RABBIT) Antibody - 600-401-D73
Dual specificity mitogen-activated protein kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1
Rabbit
Polyclonal
IgG

Target Details

MAP2K1  - View All MAP2K1 Products
Human, Rat
Phosphorylation
Conjugated Peptide
Anti-MEK1 pT386 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr 386 conjugated to KLH.
Anti-MEK1 pT386 antibodies are prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. The immunolabeling is completely eliminated by treatment with lambda-phosphatase. Expect reactivity with the following species based on sequence homology: bovine, canine, chicken, mouse, non-human primates and Xenopus. Cross reactivity with MEK1 pT386 from other species has not been determined.
Q02750 - UniProtKB
AAI39730.1 - NCBI Protein

Application Details

WB
Anti-MEK1 pT386 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 45 kDa in size corresponding to the MEK1 protein phosphorylated at Thr386 in the appropriate cell lysate or extract.

Formulation

0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.