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Datasheet

Mcl-1 Antibody

Rabbit Polyclonal

85 References
600-401-394S 600-401-394
25 µL 100 µg
Liquid (sterile filtered) Liquid (sterile filtered)
WB, ELISA, IHC, IF, IP
Mouse
Rabbit
$125.00 /Per Item
$475.00 /Per Item
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Background

Regulated apoptosis is essential for both the development and the subsequent maintenance of the immune system. Interleukins, including IL-2, IL-4, IL-7 and IL-15, heavily influence lymphocyte survival during the vulnerable stages of VDJ rearrangement and later in ensuring cellular homeostasis, but the genes specifically responsible for the development and maintenance of lymphocytes have not been identified. The Anti apoptotic protein Mcl-1 (myeloid cell leukemia sequence 1 (BCL2-related)) is an attractive candidate, as it is highly regulated, appears to enhance short-term survival and functions at an apical step in genotoxic deaths. However, Mcl-1 deficiency results in peri-implantation lethality. Mice, conditional for Mcl-1, display a profound reduction in B and T lymphocytes when Mcl-1 is removed. Deletion of Mcl-1 during early lymphocyte differentiation increases apoptosis and arrests the development at pro-B-cell and double negative T-cell stages. Induced deletion of Mcl-1 in peripheral B- and T-cell populations results in their rapid loss. Moreover, IL-7 both induces and requires Mcl-1 to mediate lymphocyte survival. Mcl-1 is essential both early in lymphoid development and later on in the maintenance of mature lymphocytes.

Product Details

Anti-Mcl-1 (RABBIT) Antibody - 600-401-394
rabbit anti-Mcl-1 antibody, Mcl1, Mcl 1, Bcl 2 related protein EAT/mcl1 antibody, Bcl2 related antibody, EAT antibody, Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
Rabbit
Polyclonal
IgG

Target Details

Mcl1  - View All Mcl1 Products
Mouse
Conjugated Peptide
This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Mcl-1 conjugated to Keyhole Limpet Hemocyanin (KLH).
This affinity-purified antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Application Details

ELISA, WB
IF, IHC, IP  - View References
Anti-Mcl-1 Antibody has been tested by ELISA and western blot and is suitable for immunoprecipitation.  This antibody detects mouse Mcl-1 and is not expected to cross react with the human sequence.  Cross reactivity with Mcl-1 from other sources is unknown.  This antibody is highly specific, showing no bands in null cell lysates from Mcl-1 knockout mice even when grossly over-exposed. Mouse Mcl-1 (myeloid cell leukemia sequence 1) is composed of 331 amino acids and is reported to be 35.2 kDa in size.  The human ortholog consists of 351 amino acids and is reported to be 37.3 kDa in size.

Formulation

1.1mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
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Applications
WB, IB, PCA
(). Chronic spindle assembly checkpoint activation causes myelosuppression and gastrointestinal atrophy. EMBO Rep.
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WB, IB, PCA
(). The SARS-CoV-2 spike glycoprotein interacts with MAO-B and impairs mitochondrial energetics. Curr Res Neurobiol.
Applications
WB, IB, PCA
(). Porphyromonas gingivalis Peptidyl Arginine Deiminase (PPAD) in the Context of the Feed-Forward Loop of Inflammation in Periodontitis. Int J Mol Sci.
Applications
WB, IB, PCA
(). Mitochondrial E3 ubiquitin ligase MARCHF5 controls BAK apoptotic activity independently of BH3-only proteins. Cell Death Differ.
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WB, IB, PCA
(). Mitophagy restricts BAX/BAK-independent, Parkin-mediated apoptosis. Sci Adv.
Applications
WB, IB, PCA
(). Mcl-1 Differentially Regulates Autophagy in Response to Changes in Energy Status and Mitochondrial Damage. Cells
Applications
WB, IB, PCA
(). MCL-1 is a master regulator of cancer dependency on fatty acid oxidation. Cell Rep.
Applications
WB, IB, PCA
(). Ca2+-mediated mitochondrial inner membrane permeabilization induces cell death independently of Bax and Bak. Cell Death Differ.
Applications
WB, IB, PCA
(). p53-Independent Induction of p21 Fails to Control Regeneration and Hepatocarcinogenesis in a Murine Liver Injury Model. Cell Mol Gastroenterol Hepatol.
Applications
WB, IB, PCA
(). Arf1 and Arf6 synergistically maintain survival of T cells during activation. J Immunol.
Applications
Undefined
(). Inhibition of the Anti-Apoptotic Bcl-2 Family by BH3 Mimetics Sensitize the Mitochondrial Permeability Transition Pore Through Bax and Bak. Frontiers in Cell and Developmental Biology
Applications
WB, IB, PCA
(). Gab1 in livers with persistent hepatocyte apoptosis has an antiapoptotic effect and reduces chronic liver injury, fibrosis, and tumorigenesis. Am J Physiol Gastrointest Liver Physiol.
Applications
Undefined
(). Ser9 phosphorylation of GSK-3β promotes aging in the heart through suppression of autophagy. J Cardiovasc Aging
Applications
WB, IB, PCA
(). The Role of Mcl-1 in Embryonic Neural Precursor Cell Apoptosis. Front Cell Dev Biol.
Applications
WB, IB, PCA
(). Requirement for Anti-Apoptotic MCL-1 during Early Erythropoiesis. Blood
Applications
WB, IB, PCA; IHC, ICC, Histology
(). Heme-Sensing Pathway Modulates Susceptibility of Poor Prognosis B-Lineage Acute Leukemia to BH3-Mimetics. Mol Cancer Res.
Applications
WB, IB, PCA
(). MCL-1 is essential for survival but dispensable for metabolic fitness of FOXP3+ regulatory T cells. Cell Death Differ.
Applications
WB, IB, PCA
(). Mcl-1-mediated mitochondrial fission protects against stress but impairs cardiac adaptation to exercise. J Mol Cell Cardiol.
Applications
IP, Co-IP; WB, IB, PCA
(). Splicing factor YBX1 mediates persistence of JAK2-mutated neoplasms. Nature.
Applications
WB, IB, PCA
(). MARCH5 requires MTCH2 to coordinate proteasomal turnover of the MCL1:NOXA complex. Cell Death and Differentiation
Applications
WB, IB, PCA
(). Hepatocyte apoptosis is tumor promoting in murine nonalcoholic steatohepatitis. Cell Death & Disease
Applications
WB, IB, PCA
(). Glucocorticoids can induce BIM to trigger apoptosis in the absence of BAX and BAK1. Cell Death Dis.
Applications
WB, IB, PCA
(). DNase II activated by the mitochondrial apoptotic pathway regulates RIP1-dependent non-apoptotic hepatocyte death via the TLR9/IFN-β signaling pathway. Cell Death Differ.
Applications
WB, IB, PCA
(). Mcl-1 and Bcl-xL are essential for survival of the developing nervous system. Cell Death Differ.
Applications
WB, IB, PCA
(). Impaired TFEB-mediated lysosomal biogenesis promotes the development of pancreatitis in mice and is associated with human pancreatitis. Autophagy.
Applications
WB, IB, PCA
(). CHK1 dosage in germinal center B cells controls humoral immunity. Cell Death Differ.
Applications
WB, IB, PCA
(). The AKT isoforms 1 and 2 drive B cell fate decisions during the germinal center response. Life Science Alliance
Applications
WB, IB, PCA
(). In the absence of apoptosis, myeloid cells arrest when deprived of growth factor, but remain viable by consuming extracellular glucose. Cell Death and Differentiation
Applications
WB, IB, PCA
(). Requirement of GSK-3 for PUMA induction upon loss of pro-survival PI3K signaling. Cell Death & Disease
Applications
WB, IB, PCA
(). Precision Targeting of BFL-1/A1 and an ATM Co-dependency in Human Cancer. Cell Reports
Applications
WB, IB, PCA
(). Infection with flaviviruses requires BCLXL for cell survival. PLOS Pathogens
Applications
WB, IB, PCA
(). Differential effects of Vav‐promoter‐driven overexpression of BCLX and BFL1 on lymphocyte survival and B cell lymphomagenesis. FEBS J.
Applications
WB, IB, PCA
(). Dynamic regulation of long-chain fatty acid oxidation by a noncanonical interaction between the MCL-1 BH3 helix and VLCAD. Mol Cell.
Applications
WB, IB, PCA
(). Bid maintains mitochondrial cristae structure and function and protects against cardiac disease in an integrative genomics study. ELife
Applications
WB, IB, PCA
(). MYC selects against reduced BCL2A1/A1 protein expression during B cell lymphomagenesis. Oncogene
Applications
WB, IB, PCA
(). The BCL-2 pro-survival protein A1 is dispensable for T cell homeostasis on viral infection. Cell Death & Differentiation
Applications
WB, IB, PCA
(). Dynein light chain 1 induces assembly of large Bim complexes on mitochondria that stabilize Mcl-1 and regulate apoptosis. Genes & Development
Applications
WB, IB, PCA
(). JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins. Cell Reports
Applications
WB, IB, PCA
(). Post-translational control of T cell development by the ESCRT protein CHMP5. Nat Immunol.
Applications
Undefined
(). Conversion of Bim-BH3 from activator to inhibitor of Bak through structure-based design. Mol Cell.
Applications
Undefined
(). The BH3-only protein BIM contributes to late-stage involution in the mouse mammary gland. Cell Death & Differentiation
Applications
WB, IB, PCA
(). Conditional knockdown of BCL2A1 reveals rate-limiting roles in BCR-dependent B-cell survival. Cell Death & Differentiation
Applications
WB, IB, PCA
(). Increased hepatic receptor interacting protein kinase 3 expression due to impaired proteasomal functions contributes to alcohol-induced steatosis and liver injury. Oncotarget
Applications
WB, IB, PCA
(). Inactivation of nuclear GSK3β by Ser(389) phosphorylation promotes lymphocyte fitness during DNA double-strand break response. Nature Communications
Applications
IF, Confocal Microscopy; WB, IB, PCA
(). The anti-apoptotic Bcl-2 family protein A1/Bfl-1 regulates neutrophil survival and homeostasis and is controlled via PI3K and JAK/STAT signaling. Cell Death & Disease
Applications
WB, IB, PCA
(). Bok Is Not Pro-Apoptotic But Suppresses Poly ADP-Ribose Polymerase-Dependent Cell Death Pathways and Protects against Excitotoxic and Seizure-Induced Neuronal Injury. The Journal of Neuroscience
Applications
WB, IB, PCA
(). Allosteric inhibition of antiapoptotic MCL-1. Nature Structural & Molecular Biology
Applications
WB, IB, PCA
(). Anti-apoptotic Bcl-XL but not Mcl-1 contributes to protection against virus-induced apoptosis. Cell Death & Disease
Applications
WB, IB, PCA
(). Structure-function insights reveal the human ribosome as a cancer target for antibiotics. Nature Communications
Applications
WB, IB, PCA
(). Cycloheximide Can Induce Bax/Bak Dependent Myeloid Cell Death Independently of Multiple BH3-Only Proteins. PLOS One
Applications
WB, IB, PCA
(). Chronic Deletion and Acute Knockdown of Parkin Have Differential Responses to Acetaminophen-induced Mitophagy and Liver Injury in Mice. Journal of Biological Chemistry
Applications
WB, IB, PCA
(). Mcl-1 is a key regulator of the ovarian reserve. Cell Death & Disease
Applications
IF, Confocal Microscopy; IHC, ICC, Histology; WB, IB, PCA
(). An interconnected hierarchical model of cell death regulation by the BCL-2 family. Nature Cell Biology
Applications
WB, IB, PCA
(). Distinct transcriptomic features are associated with transitional and mature B-cell populations in the mouse spleen. Front Immunol.
Applications
WB, IB, PCA
(). Cyclin E/Cdk2-dependent phosphorylation of Mcl-1 determines its stability and cellular sensitivity to BH3 mimetics. Oncotarget
Applications
WB, IB, PCA
(). Bax targets mitochondria by distinct mechanisms before or during apoptotic cell death: a requirement for VDAC2 or Bak for efficient Bax apoptotic function. Cell Death Differ.
Applications
WB, IB, PCA
(). HTLV-1 tax stabilizes MCL-1 via TRAF6-dependent K63-linked polyubiquitination to promote cell survival and transformation. PLoS Pathog.
Applications
IP, Co-IP; WB, IB, PCA
(). BH3-only protein BIM mediates heat shock-induced apoptosis. PloS One
Applications
WB, IB, PCA
(). Trim17-mediated ubiquitination and degradation of Mcl-1 initiate apoptosis in neurons. Cell Death Differ.
Applications
WB, IB, PCA
(). Loss of MCL-1 leads to impaired autophagy and rapid development of heart failure. Genes Dev.
Applications
WB, IB, PCA
(). Mcl1 regulates the terminal mitosis of neural precursor cells in the mammalian brain through p27Kip1. Development.
Applications
WB, IB, PCA
(). RAF and antioxidants prevent cell death induction after growth factor abrogation through regulation of Bcl-2 proteins. Exp Cell Res.
Applications
WB, IB, PCA
(). Obesity promotes liver carcinogenesis via Mcl-1 stabilization independent of IL-6Rα signaling. Cell Rep.
Applications
WB, IB, PCA
(). Regulation of cell survival by sphingosine-1-phosphate receptor S1P1 via reciprocal ERK-dependent suppression of Bim and PI-3-kinase/protein kinase C-mediated upregulation of Mcl-1. Cell Death Dis.
Applications
WB, IB, PCA
(). miR-181 targets multiple Bcl-2 family members and influences apoptosis and mitochondrial function in astrocytes. Mitochondrion.
Applications
WB, IB, PCA
(). Alternative splicing of Bim and Erk-mediated Bim EL phosphorylation are dispensable for hematopoietic homeostasis in vivo. Cell Death Differ.
Applications
WB, IB, PCA
(). Mcl-1 regulates the survival of adult neural precursor cells. Mol Cell Neurosci.
Applications
WB, IB, PCA
(). Oncogenic kit triggers Shp2/Erk1/2 pathway to down-regulate the pro-apoptotic protein Bim and to promote apoptosis resistance in leukemic cells. PLoS One
Applications
WB, IB, PCA
(). BH3 domains other than Bim and Bid can directly activate Bax/Bak. J Biol Chem.
Applications
IP, Co-IP; WB, IB, PCA
(). In vivo activity of combined PI3K/mTOR and MEK inhibition in a KrasG12D; Pten deletion mouse model of ovarian cancer. Mol Cancer Ther.
Applications
WB, IB, PCA
(). A cooperative microRNA-tumor suppressor gene network in acute T-cell lymphoblastic leukemia (T-ALL). Nat Genet.
Applications
WB, IB, PCA
(). Role of STAT5 in controlling cell survival and immunoglobulin gene recombination during pro-B cell development. Nat Immunol.
Applications
WB, IB, PCA
(). c-Myb promotes the survival of CD4+ CD8+ double-positive thymocytes through upregulation of Bcl-xL. J Immunol.
Applications
WB, IB, PCA
(). Mitophagy in mammalian cells: the reticulocyte model. Methods Enzymol.
Applications
WB, IB, PCA
(). Mcl-1 degradation during hepatocyte lipoapoptosis. J Biol Chem.
Applications
WB, IB, PCA
(). Defining the target specificity of ABT-737 and synergistic antitumor activities in combination with histone deacetylase inhibitors. Blood.
Applications
WB, IB, PCA
(). Mcl-1 and Bcl-xL cooperatively maintain integrity of hepatocytes in developing and adult murine liver. Hepatology (Baltimore, Md.)
Applications
WB, IB, PCA
(). BH3-only protein bid participates in the Bcl-2 network in healthy liver cells. Hepatology (Baltimore, Md.)
Applications
WB, IB, PCA
(). Triggering of apoptosis by Puma is determined by the threshold set by prosurvival Bcl-2 family proteins. J Mol Biol.
Applications
WB, IB, PCA
(). The anti-apoptotic Bcl-2 family member Mcl-1 promotes T lymphocyte survival at multiple stages. Journal of Immunology (Baltimore, Md. : 1950)
Applications
FC, FACS, FLOW; WB, IB, PCA
(). Interleukin 15–mediated survival of natural killer cells is determined by interactions among Bim, Noxa and Mcl-1. Nat Immunol.
Applications
WB, IB, PCA
(). NIX is required for programmed mitochondrial clearance during reticulocyte maturation. Proc Natl Acad Sci USA
Applications
WB, IB, PCA
(). Proapoptotic BH3-only BCL-2 family protein BIM connects death signaling from epidermal growth factor receptor inhibition to the mitochondrion. Cancer Res.
Applications
WB, IB, PCA
(). Dominant negative retinoic acid receptor initiates tumor formation in mice. Mol Cancer
Applications
IHC, ICC, Histology

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