Malate Dehydrogenase Antibody
Western Blot using Anti-Malate Dehydrogenase (Pig Heart) (SHEEP) Antibody
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Datasheet

Malate Dehydrogenase Antibody

Sheep Polyclonal

14 References
100-601-145
2 mL
WB, Other
Pig
Sheep
$1,225.00 /Per Item
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Background

Catalytic activity of Malate Dehydrogenase is (S)-malate + NAD+ = oxaloacetate + NADH.

Product Details

Anti-Malate Dehydrogenase (Pig Heart) (SHEEP) Antibody - 100-601-145
sheep anti-Malate Dehydrogenase Antibody, Malate dehydrogenase cytoplasmic, Cytosolic malate dehydrogenase
Sheep
Polyclonal
Antiserum

Target Details

MDH1  - View All MDH1 Products
Pig
Native Protein
Malate Dehydrogenase [Pig Heart]
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Malate Dehydrogenase [Pig Heart].  Cross reactivity against Malate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
UniProtKB - P11708
NCBI - P11708.4
GeneID - 396894

Application Details

Other, WB  - View References
This product has been assayed against 1.0 ug of Malate Dehydrogenase [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.

Formulation

Lyophilized
80 mg/mL by Refractometry
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
2.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
(). Quantification of biomarkers for beef meat qualities using a combination of Parallel Reaction Monitoring-and antibody-based proteomics. Food Chem.
Applications
Other
(). Protein Array-Based Approach to Evaluate Biomarkers of Beef Tenderness and Marbling in Cows: Understanding of the Underlying Mechanisms and Prediction. Foods.
Applications
Other; WB, IB, PCA
(). Beef tenderness and intramuscular fat proteomic biomarkers: Effect of gender and rearing practices. J Proteomics.
Applications
Other; WB, IB, PCA
(). Beef tenderness and intramuscular fat proteomic biomarkers: muscle type effect. PeerJ
Applications
WB, IB, PCA
(). Reverse phase protein arrays for the identification/validation of biomarkers of beef texture and their use for early classification of carcasses. Food Chem.
Applications
Other; WB, IB, PCA
(). Reverse Phase Protein array for the quantification and validation of protein biomarkers of beef qualities: The case of meat color from Charolais breed. Meat Sci.
Applications
Other; WB, IB, PCA
(). The study of protein biomarkers to understand the biochemical processes underlying beef color development in young bulls. Meat Sci.
Applications
Dot Blot Assay
(). Calcium Homeostasis and Muscle Energy Metabolism Are Modified in HspB1-Null Mice. Proteomes
Applications
WB, IB, PCA
(). Understanding Early Post-Mortem Biochemical Processes Underlying Meat Color and pH Decline in the Longissimus thoracis Muscle of Young Blond d'Aquitaine Bulls Using Protein Biomarkers. J Agric Food Chem.
Applications
WB, IB, PCA
(). Coherent correlation networks among protein biomarkers of beef tenderness: What they reveal. J Proteomics.
Applications
Undefined
(). Inverse relationships between biomarkers and beef tenderness according to contractile and metabolic properties of the muscle. J Agric Food Chem.
Applications
WB, IB, PCA
(). Functional analysis of beef tenderness. J Proteomics.
Applications
WB, IB, PCA
(). Variations in the abundance of 24 protein biomarkers of beef tenderness according to muscle and animal type. Animal.
Applications
WB, IB, PCA
(). Oxidative modification of proteins in the frontal cortex of Alzheimer's disease brain. Neurobiol Aging.
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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