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Datasheet

Lactate Dehydrogenase Antibody

Goat Polyclonal

9 References
100-1173
2 mL
Lyophilized
WB, ELISA
Human
Goat
$1,225.00 /Per Item
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Background

Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle.  Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a ''flipped pattern'') suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown.  LDH shows a cytoplasmic localization.

Product Details

Anti-Lactate Dehydrogenase (Rabbit Muscle) (GOAT) Antibody - 100-1173
goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M
Goat
Polyclonal
Antiserum

Target Details

LDHA  - View All LDHA Products
Human
Native Protein
This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle.
This product was prepared from monospecific antiserum by delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].  BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
P13491 - UniProtKB
126050 - NCBI Protein
100009107 - Gene ID

Application Details

ELISA
WB  - View References
This antibody has been tested in ELISA and suitable for western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 36 kDa in size corresponding to LDH western blotting in the appropriate cell lysate or extract.

Formulation

82 mg/mL by Refractometry
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
2.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.