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Datasheet

Involucrin Antibody

Mouse Monoclonal SY5 IgG1

200-301-P39
100 µg
WB, IHC
Human, Pig
Mouse
$485.00 /Per Item
Availability: Ships in approximately 14 days
Shipping info:

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Background

Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585 amino acids, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene, encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated. This antibody is suitable for researchers interested in skin cell research.

Product Details

Anti-Involucrin (MOUSE) Antibody - 200-301-P39
INVO, IVL, Involucrin Antibody
Mouse
Monoclonal
IgG1

Target Details

IVL  - View All IVL Products
Human, Pig
Other
Involucrin antibody was produced in mice by repeated immunizations with human involucrin.
Anti-Involucrin antibody was purified from mouse ascites by protein A chromatography.  This product reacts with human and pig. Cross reactivity with Involucrin from other sources has not been determined.

Application Details

IHC, WB
Anti-Involucrin is tested for Immunohistochemistry -P, -F, Immunocytochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~68.5 kDa corresponding to the appropriate cell lysate or extract.

Formulation

Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
1.2% sodium acetate
0.01% (w/v) Sodium Azide
2mg BSA
1.0 mL
Neutral PBS

Shipping & Handling

Ambient
Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.