IL-10 Antibody
Rabbit anti-IL-10 WB
Rockland Anti IL-10 Antibody
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Datasheet

IL-10 Antibody

Rabbit Polyclonal

109-401-312S 109-401-312-0100 109-401-312
25 µL 100 µL 1 mL
WB
Human
Rabbit
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$99.00 /Per Item
$399.00 /Per Item
$1,102.00 /Per Item
25 µL $99.00
100 µL $399.00
1 mL $1,102.00
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Description

Background

Anti-IL-10 Antibody recognizes IL-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), that is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. IL-10 is produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kB activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract and indeed patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant interleukin 10, showing the importance of interleukin 10 for counteracting excessive immunity in the human body. Anti-IL-10 cytokine antibody is ideal for investigators involved Immunology and Signal Transduction research.

Application Note

IL-10 antiserum has been tested for use in immunoblotting. Although not tested, this antibody may be useful in radioimmunoassay, neutralizations, immunoprecipitation, ELISA and immunohistochemistry.  Reactivity in other immunoassays is unknown. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-10 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.

Purity/Specificity

The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts).   The antibody recognizes hu IL-10. The antibody will not neutralize the biological activity of murine IL-10.

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Human IL-10 (RABBIT) Antibody - 109-401-312
CSIF antibody, Cytokine Synthesis Inhibitory Factor antibody, IL 10 antibody, IL10A antibody, rabbit anti-interleukin 10 antibody, Interleukin10 antibody, MGC126450 antibody, MGC126451 antibody, TGIF antibody, rabbit anti-IL-10
IL10
WB
Human
Human
Rabbit
Polyclonal
Antiserum
Native Protein
The whole rabbit serum was prepared by repeated immunizations with human IL-10.
Liquid (sterile filtered)
Dry Ice
Expiration date is one (1) year from date of opening.
85 mg/mL by Refractometry
None
0.01% (w/v) Sodium Azide
None

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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