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Histone H3 K4me2 Antibody

Rabbit Polyclonal

50 µg
WB, IF, ChIP, Dot Blot
Human, C. elegans
$495.00 /Per Item
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$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.


H3K4me2 is a modification thought to have a role in transcriptional memory. In CD4+ T lymphocytes, H3K4Me2 is present within gene bodies regulating cellular function, but not in those of housekeeping genes, which indicates that the modification has a role in refining the tissue-specificity of expressed genes. This type of cellular identity targeting is also noted in work with human and mouse spermatozoa; the H3K4Me2 modification marks genes that are relevant in spermatogenesis. Most effects of H3K4Me2 seem to be attributed to its transcriptional activation; however, recent work also indicates that it may also play an RNA-dependent regressive role, related to the GAL-1 promoter. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics Research.

Product Details

Anti-Histone H3 [Dimethyl Lys4] (RABBIT) Antibody - 600-401-I58
rabbit anti-Histone H3 dimethyl Lys4 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K4me2

Target Details

HIST2H3C  - View All HIST2H3C Products
Human, C. elegans
Conjugated Peptide
Histone H3 [Dimethyl Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 4 of human Histone H3.2.
Anti-Histone H3 [Dimethyl Lys4] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Application Details

ChIP, Dot Blot, IF, WB
Anti-Histone H3 [Dimethyl Lys4] antibody is tested in Western Blot, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. This antibody is useful in Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.


Liquid (sterile filtered)
1.1 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
30% Glycerol

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.