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Datasheet

Histone H3 K36me2 Antibody

Rabbit Polyclonal

600-401-I87
50 µg
C. elegans; Human; Mouse
Rabbit
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Description

Background

In normal cellular conditions, H3K36Me2 represses spurious transcription, via recruitment of a deacetylase complex, Rpd3s. However, in cancer cells, overexpression of a histone demethylation enzyme (KDM8) can lead to demethylation of H3K36Me2, which then leads to inhibition of HDAC recruitment and thus erroneous transcriptional activation. Therefore, the variable role of H3K36Me2 as an epigenetic repressor or activator seems critical in cancer research. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Synonyms

rabbit anti-Histone H3 dimethyl Lys36 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K36me2

Immunogen

Histone H3 [Dimethyl Lys36] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 36 of human Histone H3.2.

Application Note

Anti-Histone H3 [Dimethyl Lys36] antibody is useful for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunocytochemistry/Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Purity/Specificity

Anti-Histone H3 [Dimethyl Lys36] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Anti-Histone H3 [Dimethyl Lys36] (RABBIT) Antibody - 600-401-I87
HIST2H3C
Human
C. elegans; Human; Mouse
Methylation
Rabbit
Unconjugated
Polyclonal
IgG
Peptide
Liquid (sterile filtered)
Dry Ice
Expiration date is one (1) year from date of opening.
0.73 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
30% Glycerol

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