Datasheet is currently unavailable. Try again or CONTACT US
Datasheet

Histone H3 K36ac Antibody

Rabbit Polyclonal

1 References
600-401-I89
50 µg
Liquid (sterile filtered)
WB, IF, Dot Blot, Multiplex
Human, C. elegans
Rabbit
$495.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Product Details

Anti-Histone H3 [ac Lys36] (RABBIT) Antibody - 600-401-I89
rabbit anti-Histone H3 Ac Lys36 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K36ac
Rabbit
Polyclonal
IgG

Target Details

HIST2H3C  - View All HIST2H3C Products
Human, C. elegans
Acetylation
Conjugated Peptide
Histone H3 [ac Lys36] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 36 of human Histone H3.2.
Anti-Histone H3 [ac Lys36] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Q71DI3 - UniProtKB
NP_001005464.1 - NCBI Protein

Application Details

Dot Blot, IF, WB, Multiplex
Anti-Histone H3 [ac Lys36] antibody is tested for Western Blot, Immunocytochemistry, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Histone H3 acetylation at lysine 36 results from the activity of GCN5, which regulates transcription. H3K36ac is usually found in the promoters of RNA polymerase II-transcribed genes. When acetylation of this lysine is replaced by methylation, the activation of transcription is eliminated. This switch-like activity seems to determine the chromatin function found in transcription units. Set2 associates with RNA polymerase II and histone H3 at lysine 36, and seems to be responsible for proper acetylation of coding regions. The co-activity of Set2 and Rpd3S is important for ensuring that transcription of intragenic sequences does not occur, and is related to the acetylation and methylation of histone H3 at lysine 36. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
(). Surprising phenotypic diversity of cancer-associated mutations of Gly 34 in the histone H3 tail. Elife.
Applications
WB, IB, PCA

Invalid lot number

If you need help finding your CoA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.