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Datasheet

HA Epitope Tag Antibody

Rabbit Polyclonal

63 References
600-401-384S 600-401-384
25 µL 100 µg
Liquid (sterile filtered) Liquid (sterile filtered)
WB, ELISA, IHC, IF, IP, Multiplex
HA-Tag
Rabbit
$125.00 /Per Item
$299.00 /Per Item
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$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein.   Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.

Product Details

Anti-HA Epitope Tag (RABBIT) Antibody - 600-401-384
rabbit anti-HA epitope tag antibody, rabbit anti-hemagglutinin antibody, rabbit anti-HA tag antibody, anti-epitope
Rabbit
Polyclonal
IgG

Target Details

HA-Tag
Conjugated Peptide
Anti-HA antibody was purified from whole rabbit serum prepared by repeated immunizations with the epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH. 
This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag.  To date, this antibody has reacted with all HA-tagged proteins tested.  In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.

Application Details

ELISA, IHC, WB
IF, IP, Multiplex  - View References
Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins.  As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope.  The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors.  This antibody has been tested by ELISA, immunohistochemistry, and western blotting against both the immunizing peptide and HA containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques.  Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.

Formulation

1.05 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). Stress granule-localized USP8 potentiates cGAS-mediated type I interferonopathies through deubiquitination of DDX3X. Cell Rep.
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WB, IB, PCA
(). HECTD3 inhibits NLRP3 inflammasome assembly and activation by blocking NLRP3-NEK7 interaction. Cell Death Dis.
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WB, IB, PCA
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WB, IB, PCA
(). SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly. Nature Communications
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(). Kinesin-directed secretion of basement membrane proteins to a subdomain of the basolateral surface in Drosophila epithelial cells. Curr Biol.
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(). Cullin 4b Complex Targets IRGM1 to Regulate Intestinal Stem Cell Stemness and Niche. Cell Death Differ.
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WB, IB, PCA
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IP, Co-IP
(). Hypothalamic astrocytes control systemic glucose metabolism and energy balance. Cell Metab.
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IF, Confocal Microscopy
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(). Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster. PloS Genetics
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WB, IB, PCA
(). SKI activates the Hippo pathway via LIMD1 to inhibit cardiac fibroblast activation. Basic Research in Cardiology
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WB, IB, PCA
(). The periphilin 1-like BFAR isoform 3 is highly expressed in transcriptionally silent oocytes and involved in RNA metabolism. Biochim Biophys Acta Mol Cell Res.
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(). C-Terminal Domain of Aquaporin-5 Is Required to Pass Its Protein Quality Control and Ensure Its Trafficking to Plasma Membrane. Int J Mol Sci.
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(). Origin and evolution of the zinc finger antiviral protein. PLoS Pathog.
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WB, IB, PCA
(). USP18 positively regulates innate antiviral immunity by promoting K63-linked polyubiquitination of MAVS. Nat Commun.
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(). USP5 attenuates NLRP3 inflammasome activation by promoting autophagic degradation of NLRP3. Autophagy.
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(). The Multifunctional Long-Distance Movement Protein of Pea Enation Mosaic Virus 2 Protects Viral and Host Transcripts from Nonsense-Mediated Decay. mBio
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E, EIA
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(). Chronic chemogenetic activation of forebrain excitatory neurons in postnatal life evokes long-lasting changes in mood-related behavior. Elife
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IF, Confocal Microscopy
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WB, IB, PCA
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IF, Confocal Microscopy
(). Marburg virus regulates the IRE1/XBP1-dependent unfolded protein response to ensure efficient viral replication. Emerg Microbes Infect.
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WB, IB, PCA
(). Flexibility in nucleic acid binding is central to APOBEC3H antiviral activity. J Virol.
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WB, IB, PCA
(). Anterograde trafficking signals in GABAA subunits are required for functional expression. Channels (Austin).
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WB, IB, PCA
(). Chemogenetic activation of excitatory neurons alters hippocampal neurotransmission in a Dose-Dependent manner. eNeuro.
Applications
IF, Confocal Microscopy
(). Regulation of somatostatin receptor 2 trafficking by C-tail motifs and the retromer. Endocrinology.
Applications
IF, Confocal Microscopy; WB, IB, PCA; Multiplex Assay
(). Stress-activated MAPKs and CRM1 regulate the subcellular localization of Net1A to control cell motility and invasion. Journal of Cell Science
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WB, IB, PCA
(). HSP90 inhibitors disrupt a transient HSP90-HSF1 interaction and identify a noncanonical model of HSP90-mediated HSF1 regulation. Scientific Reports
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(). SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death. Cell Death & Disease
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WB, IB, PCA
(). The α-arrestin ARRDC3 suppresses breast carcinoma invasion by regulating G protein–coupled receptor lysosomal sorting and signaling. J Biol Chem.
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IF, Confocal Microscopy; WB, IB, PCA
(). Protein Tyrosine Phosphatase Receptor Type G (PTPRG) Controls Fibroblast Growth Factor Receptor (FGFR) 1 Activity and Influences Sensitivity to FGFR Kinase Inhibitors. Mol Cell Proteomics.
Applications
IP, Co-IP
(). Mitochondrial stasis reveals p62-mediated ubiquitination in Parkin-independent mitophagy and mitigates nonalcoholic fatty liver disease. Cell Metab.
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IF, Confocal Microscopy; Multiplex Assay
(). S. pombe Uba1-Ubc15 Structure Reveals a Novel Regulatory Mechanism of Ubiquitin E2 Activity. Molecular Cell
Applications
WB, IB, PCA
(). A Novel Mechanism for the Grid-to-Place Cell Transformation Revealed by Transgenic Depolarization of Medial Entorhinal Cortex Layer II. Neuron
Applications
IHC, ICC, Histology
(). Co-option of an endogenous retrovirus envelope for host defense in hominid ancestors. Elife
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WB, IB, PCA
(). APOBEC3H structure reveals an unusual mechanism of interaction with duplex RNA. Nature Communications
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WB, IB, PCA
(). Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin. Frontiers in Synaptic Neuroscience
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WB, IB, PCA
(). Pharmacogenetic reactivation of the original engram evokes an extinguished fear memory. Neuropharmacology.
Applications
IF, Confocal Microscopy; Multiplex Assay
(). Time-gated detection of protein-protein interactions with transcriptional readout. Elife
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IF, Confocal Microscopy
(). Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to ApoFet3p Multi-copper Ferroxidase. Journal of Biological Chemistry
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WB, IB, PCA
(). Cadmium and Secondary Structure-dependent Function of a Degron in the Pca1p Cadmium Exporter. Journal of Biological Chemistry
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(). Endoplasmic Reticulum-associated Degradation of Pca1p, a Polytopic Protein, via Interaction with the Proteasome at the Membrane. Journal of Biological Chemistry
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WB, IB, PCA
(). The RNA Binding Specificity of Human APOBEC3 Proteins Resembles That of HIV-1 Nucleocapsid. PLOS Pathogens
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(). Cdc42p-interacting protein Bem4p regulates the filamentous-growth mitogen-activated protein kinase pathway. Molecular and Cellular Biology
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(). Impact of Adenovirus E4-ORF3 Oligomerization and Protein Localization on Cellular Gene Expression. Viruses
Applications
IF, Confocal Microscopy; WB, IB, PCA
(). Gain-of-function p53 mutants co-opt chromatin pathways to drive cancer growth. Nature
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WB, IB, PCA
(). drd2‐cre:ribotag mouse line unravels the possible diversity of dopamine d2 receptor‐expressing cells of the dorsal mouse hippocampus. Hippocampus.
Applications
IF, Confocal Microscopy; Multiplex Assay
(). YCF1-mediated cadmium resistance in yeast is dependent on copper metabolism and antioxidant enzymes. Antioxid Redox Signal.
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WB, IB, PCA
(). Impaired OMA1-dependent cleavage of OPA1 and reduced DRP1 fission activity combine to prevent mitophagy in cells that are dependent on oxidative phosphorylation. J Cell Sci.
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WB, IB, PCA
(). Kinesin-14 and kinesin-5 antagonistically regulate microtubule nucleation by γ-TuRC in yeast and human cells. Nat Commun.
Applications
WB, IB, PCA
(). Global changes in the RNA binding specificity of HIV-1 gag regulate virion genesis. Cell.
Applications
IP, Co-IP; WB, IB, PCA
(). Rho gtpase independent regulation of atm activation and cell survival by the rhogef net1a Cell Cycle
Applications
WB, IB, PCA
(). qPCA: a scalable assay to measure the perturbation of protein–protein interactions in living cells. Mol Biosyst.
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WB, IB, PCA
(). Kinesin-14 Pkl1 targets γ-tubulin for release from the γ-tubulin ring complex (γ-TuRC)‬‬‬‬‬‬‬. Cell Cycle.
Applications
WB, IB, PCA
(). Starch-binding domain-containing protein 1 (Stbd1) and glycogen metabolism: Identification of the Atg8 family interacting motif (AIM) in Stbd1 required for interaction with GABARAPL1. Biochem Biophys Res Commun.
Applications
IF, Confocal Microscopy
(). Nectin-like molecules/SynCAMs are required for post-crossing commissural axon guidance. Development.
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IHC, ICC, Histology; Multiplex Assay
(). FRS2 via fibroblast growth factor receptor 1 is required for platelet-derived growth factor receptor beta-mediated regulation of vascular smooth muscle marker gene expression. J Biol Chem.
Applications
IP, Co-IP

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