HA Epitope Tag Antibody
Anti-HA Epitope Tag Antibody - Immunohistochemistry
Anti-HA Epitope Tag Antibody - Western Blot
Western Blot of Anti-HA
Western Blot of Anti-HA
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Datasheet

HA Epitope Tag Antibody

Rabbit Polyclonal

600-401-384S 600-401-384
25 µL 100 µg
ELISA, IHC, WB, IF, IP
HA-Tag
Rabbit
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$125.00 /Per Item
$279.00 /Per Item
25 µL $125.00
100 µg $279.00
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Description

Background

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein.   Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.

Application Note

Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins.  As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope.  The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors.  This antibody has been tested by ELISA, immunohistochemistry, and western blotting against both the immunizing peptide and HA containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques.  Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.

Purity/Specificity

This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag.  To date, this antibody has reacted with all HA-tagged proteins tested.  In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.

Anti-HA EPITOPE TAG (RABBIT) Antibody - 600-401-384
rabbit anti-HA epitope tag antibody, rabbit anti-hemagglutinin antibody, rabbit anti-HA tag antibody, anti-epitope
ELISA, IHC, WB
IF, IP
HA-Tag
Rabbit
HA
Polyclonal
IgG
Peptide
Anti-HA antibody was purified from whole rabbit serum prepared by repeated immunizations with the epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH. 
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly. Nature Communications ()
Applications
WB, IB, PCA
Kinesin-directed secretion of basement membrane proteins to a subdomain of the basolateral surface in Drosophila epithelial cells. Curr Biol. ()
Applications
Undefined
Cullin 4b Complex Targets IRGM1 to Regulate Intestinal Stem Cell Stemness and Niche. Cell Death Differ. ()
Applications
WB, IB, PCA
Cdk1 phosphorylation negatively regulates the activity of Net1 towards RhoA during mitosis. Cell Signal. ()
Applications
Undefined
The PDZ domain protein SYNJ2BP regulates GRK-dependent Sst2A phosphorylation and downstream MAPK signaling. Endocrinology. ()
Applications
IF, Confocal Microscopy
Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster. PloS Genetics ()
Applications
WB, IB, PCA
SKI activates the Hippo pathway via LIMD1 to inhibit cardiac fibroblast activation. Basic Research in Cardiology ()
Applications
WB, IB, PCA
The periphilin 1-like BFAR isoform 3 is highly expressed in transcriptionally silent oocytes and involved in RNA metabolism. Biochim Biophys Acta Mol Cell Res. ()
Applications
Undefined
C-Terminal Domain of Aquaporin-5 Is Required to Pass Its Protein Quality Control and Ensure Its Trafficking to Plasma Membrane. Int J Mol Sci. ()
Applications
Undefined
Origin and evolution of the zinc finger antiviral protein. PLoS Pathog. ()
Applications
WB, IB, PCA
USP18 positively regulates innate antiviral immunity by promoting K63-linked polyubiquitination of MAVS. Nat Commun. ()
Applications
WB, IB, PCA
USP5 attenuates NLRP3 inflammasome activation by promoting autophagic degradation of NLRP3. Autophagy. ()
Applications
Undefined
The Multifunctional Long-Distance Movement Protein of Pea Enation Mosaic Virus 2 Protects Viral and Host Transcripts from Nonsense-Mediated Decay. mBio ()
Applications
WB, IB, PCA
Interplay of septin amphipathic helices in sensing membrane-curvature and filament bundling. bioRxiv ()
Applications
WB, IB, PCA
Genetically engineered cell-derived nanoparticles for targeted breast cancer immunotherapy. Mol Ther. ()
Applications
E, EIA
Dopamine D2-Like Receptors Modulate Intrinsic Properties and Synaptic Transmission of Parvalbumin Interneurons in the Mouse Primary Motor Cortex. eNeuro. ()
Applications
IF, Confocal Microscopy
The switch-like expression of heme-regulated kinase 1 mediates neuronal proteostasis following proteasome inhibition. Elife ()
Applications
IF, Confocal Microscopy
A Chemogenetic Tool that Enables Functional Neural Circuit Analysis. Cell Rep. ()
Applications
IHC, ICC, Histology
Chronic chemogenetic activation of forebrain excitatory neurons in postnatal life evokes long-lasting changes in mood-related behavior. Elife ()
Applications
IF, Confocal Microscopy
Suppression of canonical TGF-β signaling enables GATA4 to interact with H3K27me3 demethylase JMJD3 to promote cardiomyogenesis. J Mol Cell Cardiol. ()
Applications
WB, IB, PCA
Planar-polarized semaphorin-5c and plexin A promote the collective migration of epithelial cells in Drosophila. Curr Biol. ()
Applications
IF, Confocal Microscopy
Marburg virus regulates the IRE1/XBP1-dependent unfolded protein response to ensure efficient viral replication. Emerg Microbes Infect. ()
Applications
WB, IB, PCA
Flexibility in nucleic acid binding is central to APOBEC3H antiviral activity. J Virol. ()
Applications
WB, IB, PCA
Anterograde trafficking signals in GABAA subunits are required for functional expression. Channels (Austin). ()
Applications
WB, IB, PCA
Chemogenetic activation of excitatory neurons alters hippocampal neurotransmission in a Dose-Dependent manner. eNeuro. ()
Applications
IF, Confocal Microscopy
Regulation of somatostatin receptor 2 trafficking by C-tail motifs and the retromer. Endocrinology. ()
Applications
IF, Confocal Microscopy
Regulation of somatostatin receptor 2 trafficking by C-tail motifs and the retromer. Endocrinology. ()
Applications
IF, Confocal Microscopy; WB, IB, PCA
Stress-activated MAPKs and CRM1 regulate the subcellular localization of Net1A to control cell motility and invasion. Journal of Cell Science ()
Applications
WB, IB, PCA
HSP90 inhibitors disrupt a transient HSP90-HSF1 interaction and identify a noncanonical model of HSP90-mediated HSF1 regulation. Scientific Reports ()
Applications
WB, IB, PCA
SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death. Cell Death & Disease ()
Applications
WB, IB, PCA
The α-arrestin ARRDC3 suppresses breast carcinoma invasion by regulating G protein–coupled receptor lysosomal sorting and signaling. J Biol Chem. ()
Applications
IF, Confocal Microscopy; WB, IB, PCA
Protein Tyrosine Phosphatase Receptor Type G (PTPRG) Controls Fibroblast Growth Factor Receptor (FGFR) 1 Activity and Influences Sensitivity to FGFR Kinase Inhibitors. Mol Cell Proteomics. ()
Applications
IP, Co-IP
Mitochondrial stasis reveals p62-mediated ubiquitination in Parkin-independent mitophagy and mitigates nonalcoholic fatty liver disease. Cell Metab. ()
Applications
IF, Confocal Microscopy
S. pombe Uba1-Ubc15 Structure Reveals a Novel Regulatory Mechanism of Ubiquitin E2 Activity. Molecular Cell ()
Applications
WB, IB, PCA
A Novel Mechanism for the Grid-to-Place Cell Transformation Revealed by Transgenic Depolarization of Medial Entorhinal Cortex Layer II. Neuron ()
Applications
IHC, ICC, Histology
Co-option of an endogenous retrovirus envelope for host defense in hominid ancestors. Elife ()
Applications
WB, IB, PCA
APOBEC3H structure reveals an unusual mechanism of interaction with duplex RNA. Nature Communications ()
Applications
WB, IB, PCA
Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin. Frontiers in Synaptic Neuroscience ()
Applications
WB, IB, PCA
Pharmacogenetic reactivation of the original engram evokes an extinguished fear memory. Neuropharmacology. ()
Applications
IF, Confocal Microscopy
Time-gated detection of protein-protein interactions with transcriptional readout. Elife ()
Applications
IF, Confocal Microscopy
Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to ApoFet3p Multi-copper Ferroxidase. Journal of Biological Chemistry ()
Applications
WB, IB, PCA
Cadmium and Secondary Structure-dependent Function of a Degron in the Pca1p Cadmium Exporter. Journal of Biological Chemistry ()
Applications
WB, IB, PCA
Endoplasmic Reticulum-associated Degradation of Pca1p, a Polytopic Protein, via Interaction with the Proteasome at the Membrane. Journal of Biological Chemistry ()
Applications
WB, IB, PCA
The RNA Binding Specificity of Human APOBEC3 Proteins Resembles That of HIV-1 Nucleocapsid. PLOS Pathogens ()
Applications
WB, IB, PCA
Cdc42p-interacting protein Bem4p regulates the filamentous-growth mitogen-activated protein kinase pathway. Molecular and Cellular Biology ()
Applications
WB, IB, PCA
Impact of Adenovirus E4-ORF3 Oligomerization and Protein Localization on Cellular Gene Expression. Viruses ()
Applications
IF, Confocal Microscopy; WB, IB, PCA
Gain-of-function p53 mutants co-opt chromatin pathways to drive cancer growth. Nature ()
Applications
WB, IB, PCA
drd2‐cre:ribotag mouse line unravels the possible diversity of dopamine d2 receptor‐expressing cells of the dorsal mouse hippocampus. Hippocampus. ()
Applications
Undefined
YCF1-mediated cadmium resistance in yeast is dependent on copper metabolism and antioxidant enzymes. Antioxid Redox Signal. ()
Applications
WB, IB, PCA
Impaired OMA1-dependent cleavage of OPA1 and reduced DRP1 fission activity combine to prevent mitophagy in cells that are dependent on oxidative phosphorylation. J Cell Sci. ()
Applications
WB, IB, PCA
Kinesin-14 and kinesin-5 antagonistically regulate microtubule nucleation by γ-TuRC in yeast and human cells. Nat Commun. ()
Applications
WB, IB, PCA
Global changes in the RNA binding specificity of HIV-1 gag regulate virion genesis. Cell. ()
Applications
IP, Co-IP; WB, IB, PCA
Rho gtpase independent regulation of atm activation and cell survival by the rhogef net1a Cell Cycle ()
Applications
WB, IB, PCA
qPCA: a scalable assay to measure the perturbation of protein–protein interactions in living cells. Mol Biosyst. ()
Applications
Undefined
Kinesin-14 Pkl1 targets γ-tubulin for release from the γ-tubulin ring complex (γ-TuRC)‬‬‬‬‬‬‬. Cell Cycle. ()
Applications
WB, IB, PCA
Starch-binding domain-containing protein 1 (Stbd1) and glycogen metabolism: Identification of the Atg8 family interacting motif (AIM) in Stbd1 required for interaction with GABARAPL1. Biochem Biophys Res Commun. ()
Applications
IF, Confocal Microscopy
Nectin-like molecules/SynCAMs are required for post-crossing commissural axon guidance. Development. ()
Applications
IHC, ICC, Histology
FRS2 via fibroblast growth factor receptor 1 is required for platelet-derived growth factor receptor beta-mediated regulation of vascular smooth muscle marker gene expression. J Biol Chem. ()
Applications
IP, Co-IP

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