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GRP78 Antibody
Mouse Monoclonal 6H4-2G7 IgG1
1 References
200-301-F37
100 µg
Liquid (sterile filtered)
WB, IF
Human, Mouse, Rat, Bovine, Cryphonectria parasitica, Hamster, Monkey, Rabbit, Xenopus
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-GRP78 (MOUSE) Monoclonal Antibody - 200-301-F37
BiP, Grp78, HspA5, MIF2, immunoglobulin heavy chain binding protein, 78 kDa glucose-regulated protein, Heat shock 70 kDa protein 5
Mouse
Monoclonal
IgG1
Target Details
HSPA5 - View All HSPA5 Products
Human, Mouse, Rat, Bovine, Cryphonectria parasitica, Hamster, Monkey, Rabbit, Xenopus
Recombinant Protein
Grp78 Antibody was produced in mice by repeated immunizations with a his-tagged human GRP78.
Anti-GRP78 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with GRP78 from Human, Mouse, Rat, Cow, Cryphonectria parasitica, Hamster, Monkey, Rabbit, and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with GRP78 from other sources has not been determined. Heat Shock research.
P11021 - UniProtKB
NP_005338.1 - NCBI Protein
Application Details
IF, WB
Anti-GRP78 Antibody has been tested in WB and IF/ICC. Expect a band approximately ~78kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.
Formulation
1mg/mL by UV absorbance at 280 nm
1X PBS, pH 7.4
0.09% (w/v) Sodium Azide
50% (v/v) Glycerol
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
GRP78 is a ubiquitously expressed, 78-kDa glucose- regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5´ nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed. GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis. Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival.
In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress, and its protein levels are reduced in the brains of Alzheimer’s patients. Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs.
Hong AL et al. (2019). Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition. eLife.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
