GFP Antibody Fluorescein Conjugated
GFP Antibody Fluorescein Conjugated Western Blot
GFP Antibody Fluorescein Conjugated - Immunofluorescence
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Datasheet

GFP Antibody Fluorescein Conjugated

Rabbit Polyclonal

600-402-215
100 µg
Dot Blot, WB, FC, IHC
eGFP, rGFP
Rabbit
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Description

Background

Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

Application Note

Anti-Green fluorescent protein Fluorescein conjugated Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Purity/Specificity

GFP Antibody Fluorescein Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum.  No reaction was observed against Human, Mouse and Rat Serum Proteins.

Anti-GFP (RABBIT) Antibody Fluorescein Conjugated - 600-402-215
rabbit anti-GFP antibody fluorescein conjugation, FITC conjugated rabbit anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Dot Blot, WB
FC, IHC
 - View References
eGFP, rGFP
Rabbit
GFP
Fluorescein (FITC)
Polyclonal
IgG
Recombinant Protein
Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
7.2 moles FITC per mole of IgG
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Lee RD et al. Single-cell analysis identifies dynamic gene expression networks that govern B cell development and transformation. Nat Commun. (2021)
Applications
FC, FACS, FLOW
PubMed
Dolina JS, Lee J, Griswold RQ, et al. TLR9 Sensing of Self-DNA Controls Cell-Mediated Immunity to Listeria Infection via Rapid Conversion of Conventional CD4+ T Cells to Treg. Cell Rep. (2020)
Applications
FC, FACS, FLOW
PubMed
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Applications
IF, Confocal Microscopy
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Ogawa C et al. Blimp-1 functions as a molecular switch to prevent inflammatory activity in Foxp3+ RORγt+ regulatory T cells. Cell Rep. (2018)
Applications
FC, FACS, FLOW
PubMed
Bankoti et al. Differential regulation of Effector and Regulatory T cell function by Blimp1. Scientific Reports (2017)
Applications
FC, FACS, FLOW
PubMed
Collins, CM et al. Tracking murine gammaherpesvirus 68 infection of germinal center B cells in vivo. PloS One (2012)
Applications
IF, Confocal Microscopy
PubMed
Guo F et al. Macroglial plasticity and the origins of reactive astroglia in experimental autoimmune encephalomyelitis. J Neurosci. (2011)
Applications
IHC, ICC, Histology
PubMed
Sokol CL et al. A mechanism for the initiation of allergen-induced T helper type 2 responses. Nat Immunol. (2008)
Applications
IHC, ICC, Histology
PubMed

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.