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Datasheet SDS

Fibronectin Antibody

Rabbit Polyclonal

5 References
600-401-117-0.1 600-401-117-0.5
100 µg 500 µg
Liquid (sterile filtered) Liquid (sterile filtered)
WB, IHC, IF
Human
Rabbit
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$949.00 /Per Item
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Background

Human fibronectin has a molecular weight of 450,000 daltons when purified in an intact form from plasma. Fibronectin is a glycoprotein synthesized in the liver for the circulating blood plasma form, and is synthesized by many mesenchymal cells, for the extracellular matrix form. It is composed of two similar, but not identical protein chains, which are linked by two disulfide linkages at the C-terminal end of the chains. The chains are composed of domains which have specific secondary structures linked together by regions which are especially susceptible to proteolytic action. For this reason, detection by immunoblot (western) may show considerable variability in the observed apparent molecular weights, which is predicated on the source of the fibronectin, and to what degree proteolysis has occurred. Bands approximately 225 kDa should be observed after SDS-PAGE when reducing and denaturing conditions are used.

Product Details

Anti-Fibronectin (Human) (RABBIT) Antibody - 600-401-117-0.1
rabbit anti-Fibronectin antibody, FN1, FN, Cold-insoluble globulin, CIG, Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3
Rabbit
Polyclonal
IgG

Target Details

FN1  - View All FN1 Products
Human
Native Protein
Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine.  The eluted material was further purified by gel filtration.  Immunization occurred after single-band purity was assessed by SDS-PAGE.
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities.  Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.  This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin.  Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.
P02751 - UniProtKB
AAA53376.1 - NCBI Protein
2335 - Gene ID

Application Details

IF, IHC, WB  - View References
Anti-Fibronectin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.  Rockland's anti-Fibronectin detects intact fibronectin (Invitrogen, Cat. No. 33016-015) by western blot after digestion by Matrix Metalloproteinase-3 (MMP-3) overnight at 37° C.  Separation was performed using a 4-12% Tris-Glycine gel.   Under these conditions a sizeable, dark band at ~220 kDa representing the undigested fibronectin, as well as many, smaller bands representing the variably sized fragments resulting from fibronectin digestion by MMP-3 were noted.  For immunohistochemistry paraffin embedded tissue preparation is recommended.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Wet Ice
Store vial at 4° C prior to opening.   This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Expiration date is one (1) year from date of receipt.
(). Epithelial-fibroblast cross talk aggravates the impact of the nephrotoxin ochratoxin A. Biochem Biophys Acta Mol Cell Res.
Applications
WB, IB, PCA
(). Intra-islet lesions and lobular variations in β-cell mass expansion in ob/ob mice revealed by 3D imaging of intact pancreas. Scientific Reports
Applications
IHC, ICC, Histology
(). The control of endothelial cell adhesion and migration by shear stress and matrix-substrate anchorage. Biomaterials.
Applications
IF, Confocal Microscopy
(). The anchorless adhesin Eap (extracellular adherence protein) from Staphylococcus aureus selectively recognizes extracellular matrix aggregates but binds promiscuously to monomeric matrix macromolecules. Matrix Biol.
Applications
WB, IB, PCA
(). Identification of Fibronectin-Binding Proteins in Mycoplasma gallisepticum Strain R. Infect Immun.
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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